Three-dimensional Recordings
Three-dimensional Recordings - Related Videos
05:40
Generation of Three-Dimensional Spheroids/Organoids from Two-Dimensional Cell Cultures Using a Novel Stamp Device
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Cited by 2 •
2025
This study presents a cost-effective and efficient methodology for generating 3D cell structures using a stamp-based system to create microwells in agarose molds. The system promotes the formation of uniform spheroids/organoids, thus improving cell interactions. This approach reduces experimental variability and supports applications in drug testing and tissue engineering.
03:26
Electroencephalography-Based Recording Technique to Record the Epileptiform Activity
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2025
This video describes the technique for recording the epileptiform activity of rhinal cortex-hippocampus organotypic slices ex vivo using electrophysiology or EEG set-up. This helps to study the dynamics and progression of epileptogenesis and screen potential therapeutic targets to treat epilepsy.
06:00
Forebrain Electrophysiological Recording in Larval Zebrafish
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Cited by 55 •
2013
A simple method to record extracellular field potentials in the larval zebrafish forebrain is described. The method provides a robust in vivo read-out of seizure-like activity. This technique can be used with genetically modified zebrafish larvae carrying epilepsy-related genes or seizures evoked by administration of convulsant drugs.
07:31
Two-Dimensional Gel Electrophoresis
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2023
Two-dimensional gel electrophoresis (2DGE) is a technique that can resolve thousands of biomolecules from a mixture. This technique involves two distinct separation methods that have been coupled together: isoelectric focusing (IEF) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). This physically separates compounds across two axes of a gel by their isoelectric points (an electrochemical property) and their molecular weights. The procedure in this video covers the main...
01:32
Three-Dimensional Culture Systems: Spheroids and Organoids
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2026
Three-dimensional (3D) culture systems, including spheroids and organoids, support New Approach Methodology (NAM)-aligned research by providing more physiologically relevant models than conventional two-dimensional cell cultures. Because they more closely resemble the structure and cellular interactions of tissues and organs, these systems can provide physiologically relevant data before advancing to animal studies. Spheroids as Disease and Drug Screening Models Spheroids are generated when one...
FAQs
Triangulation combines observations from multiple viewpoints to reconstruct an organism’s location in space. Calibration provides the spatial reference needed to relate those observations, while synchronized recordings preserve the subject’s position across time. The resulting coordinates allow researchers to analyze movement along the x, y, and z axes rather than inferring depth from a single visual perspective.
Synchronization ensures that observations from different cameras or sensors correspond to the same moment in the behavior. Without temporal alignment, apparent differences between viewpoints could reflect recording delays rather than actual movement. Coordinated timing is therefore important for measuring interactions, comparing positions, and following changes in locomotion or posture across successive observations.
A single viewpoint can obscure movement occurring toward or away from the camera, as well as changes in body position that are difficult to interpret in a flat image. Three-dimensional recordings preserve these spatial relationships, helping researchers examine posture, navigation, locomotion, and interactions with greater precision across individuals or experimental conditions.
Reconstructed coordinates support quantitative measurements of an organism’s location, movement, posture, and spatial pattern over time. Researchers can use these measurements to characterize locomotion, navigation, and interactions, then compare behavioral features across individuals or experimental conditions. This numerical representation also supports more precise analyses than relying only on qualitative visual descriptions.
A typical workflow uses synchronized cameras or sensors positioned to observe the subject from multiple viewpoints. The recording system is calibrated so observations share a spatial reference, after which the subject’s location is reconstructed through triangulation. Researchers then examine the resulting coordinates over time to quantify locomotion, posture, navigation, interactions, or spatial patterns.
Researchers would choose this approach when the behavior depends on depth, changing posture, movement through space, or relationships between individuals. It is especially useful when a single view cannot capture the full movement pattern. The measurements can support comparisons across experimental conditions and help identify behavioral features that remain difficult to detect in two-dimensional video.
In motor control and neuroscience, spatially resolved behavioral measurements connect observable actions with the organization of movement through space. Quantifying posture, locomotion, navigation, and interactions provides a more detailed behavioral outcome for comparing individuals or conditions. These data can therefore strengthen analyses that examine how organisms perform and coordinate behavior in three dimensions.