Microscopy Session
Microscopy Session - Related Videos
07:52
Cryo-Electron Microscopy Screening Automation Across Multiple Grids Using Smart Leginon
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Cited by 3 •
2023
Cryo-electron microscopy (cryoEM) multi-grid screening is often a tedious process that demands hours of attention. This protocol shows how to set up a standard Leginon collection and Smart Leginon Autoscreen to automate this process. This protocol can be applied to the majority of cryoEM holey foil grids.
09:19
Short Session High Intensity Interval Training and Treadmill Assessment in Aged Mice
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Cited by 15 •
2019
Short session (≤10 min) high intensity interval training (HIIT) is emerging as an alternative to longer exercise modalities, yet the shorter variants are rarely modeled in animal studies. Here, we describe a 10 min, 3 day a week, uphill treadmill HIIT protocol that enhances physical performance in male and female aged mice.
11:16
Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
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Cited by 12 •
2016
A method is described whereby quantum dot (QD) nanoparticles can be used for correlative immunocytochemical studies of epoxy embedded human pathology tissue. We employ commercial antibody fragment conjugated QDs that are visualized by widefield fluorescence light microscopy and transmission electron microscopy.
09:26
Experimental Screening Protocols, Immunocytochemistry and Microscopy-based Imaging Techniques for Penium margaritaceum
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2025
This report describes the fundamental methods used to culture and experimentally manipulate the unicellular streptophyte alga, Penium margaritaceum. It also provides fundamental protocols of microscopy-based imaging, including live cell labeling with monoclonal antibodies and other fluorescent probes and scanning electron microscopy.
08:57
Focussed Ion Beam Milling and Scanning Electron Microscopy of Brain Tissue
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Cited by 101 •
2011
This protocol describes how resin embedded brain tissue can be prepared and imaged in the three dimensions in the focussed ion beam, scanning electron microscope.
FAQs
Magnification enlarges the apparent size of specimen features, whereas resolution determines whether nearby or fine features can be distinguished. Increasing magnification alone does not guarantee more usable detail. During a microscopy session, researchers therefore consider both settings when examining cell structure or biomolecule localization, because poor resolution can limit biochemical interpretation even when the image appears large.
Image contrast arises when parts of a specimen produce different signals. Depending on the instrument and sample, those signals may involve light, fluorescence, or electrons; lenses or detectors then convert the differences into visible contrast. This signal-to-image relationship determines which structural or biochemical features can be distinguished during observation.
Focus and imaging settings determine whether recorded differences represent specimen features clearly enough for interpretation. An improperly focused image can obscure structure, while unsuitable settings may reduce the usefulness of contrast or signal. Controlling focus together with magnification and resolution helps investigators compare observations, assess sample quality, and document experimental changes more reliably.
Optical and electron-based instruments differ in the signal used to form the image. Optical approaches work from light-related information, including fluorescence, whereas electron-based approaches use electron signals detected from the specimen. This distinction affects how contrast is generated and helps researchers choose an imaging approach suited to the structural or biochemical feature they need to examine.
A structured microscopy session begins with sample preparation, followed by illumination or scanning and selection of suitable imaging conditions. Lenses or detectors collect the resulting signal, and the researcher adjusts magnification, focus, resolution, and other imaging settings before documenting the image. Keeping these steps organized supports consistent observation and makes later interpretation of sample differences more dependable.
Biochemists may examine cell structure, determine where biomolecules are localized, assess whether a sample is suitable for further observation, or document changes under experimental conditions. These uses connect spatial visual information with biochemical questions, allowing researchers to relate where a feature appears to its possible functional context and to compare observations across experimental conditions.
It can reveal differences in light, fluorescence, or electron signal as visual contrast, allowing researchers to inspect structure, locate biomolecules, evaluate sample quality, and record experimental changes. The value of the result depends on controlled focus, magnification, resolution, and imaging settings during acquisition, since these factors support reliable interpretation of the captured image.