Crosslinking Solution
Crosslinking Solution - Related Videos
03:47
Crosslinking Neuronal Surface Proteins in the Mouse Brain using a Chemical Crosslinking Assay
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2025
This video demonstrates the crosslinking of neuronal surface proteins using Bis-sulfosuccinimidyl suberate or BS3. Isolated and chilled mouse brain is sectioned coronally. The desired region is extracted from the sections, minced, and treated with BS3. BS3 crosslinks surface proteins like GABA receptors, leaving internal proteins unaltered. The reaction is quenched with glycine before processing the samples for further analysis.
11:15
Nanosponge Tunability in Size and Crosslinking Density
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Cited by 1 •
2017
This article describes a process for tuning the size and crosslinking density of covalently crosslinked nanoparticles from linear polyesters containing pendant functionality. By tailoring synthesis parameters (polymer molecular weight, pendant functionality incorporation, and crosslinker equivalents), a desired nanoparticle size and crosslinking density can be achieved for drug delivery applications.
09:06
Preparation of DNA-crosslinked Polyacrylamide Hydrogels
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Cited by 5 •
2014
Our laboratory has developed DNA-crosslinked polyacrylamide hydrogels, a dynamic hydrogel system, to better understand the effects of modulating tissue stiffness on cell function. Here, we provide schematics, descriptions, and protocols to prepare these hydrogels.
10:12
Quantitative Detection of DNA-Protein Crosslinks and Their Post-Translational Modifications
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Cited by 3 •
2023
The present protocol highlights a modified method to detect and quantify DNA-protein crosslinks (DPCs) and their post-translational modifications (PTMs), including ubiquitylation, SUMOylation, and ADP-ribosylation induced by topoisomerase inhibitors and by formaldehyde, thereby allowing the study of the formation and repair of DPCs and their PTMs.
05:17
BS3 Chemical Crosslinking Assay: Evaluating the Effect of Chronic Stress on Cell Surface GABAA Receptor Presentation in the Rodent Brain
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2023
The BS3 chemical crosslinking assay reveals reduced cell surface GABAA receptor expression in mouse brains under chronic psychosocial stress conditions.
FAQs
Covalent crosslinkers create chemical bonds between reactive functional groups, whereas ionic formulations rely on charge-based interactions and physical systems form networks through noncovalent interactions. These mechanisms give researchers different ways to adjust material behavior. The choice affects the resulting network’s mechanical strength, chemical stability, elasticity, and biological suitability in applications such as hydrogels and tissue scaffolds.
pH, temperature, concentration, and light exposure can control how a crosslinking formulation forms its network. Adjusting these conditions changes the extent and character of interactions among polymer chains or biomolecules. Researchers use this control to tune properties such as stiffness, porosity, swelling, degradation resistance, and compatibility for a particular bioengineering use.
The chemistry determines how the connected network responds mechanically, chemically, and biologically. A formulation may be selected to increase strength and resistance to degradation, or to produce greater elasticity, swelling, or compatibility. Because these properties are linked to the network formed under the chosen conditions, crosslinker selection is central to matching a material with its intended biomedical function.
A typical workflow begins by identifying the required material properties, then selecting a compatible crosslinking chemistry and formulation. Researchers apply the solution to polymer chains or biomolecules and control relevant conditions, including pH, temperature, concentration, or light exposure. They then assess the resulting network for properties such as stiffness, porosity, swelling, stability, and degradation resistance.
Crosslinking formulations can support the design of hydrogels, tissue scaffolds, drug-delivery systems, and biomaterial coatings. In each case, the network helps determine functional characteristics such as mechanical strength, elasticity, swelling, porosity, and resistance to degradation. Researchers can therefore adapt the formulation and reaction conditions to the performance requirements of different biomedical materials.
Researchers match the formulation and reaction conditions to the properties required by the application. A tissue scaffold may need a suitable balance of strength, elasticity, porosity, and biological compatibility, while a drug-delivery material may require controlled swelling and stability. Adjusting the crosslinking chemistry, pH, temperature, concentration, or light exposure provides a way to tune these outcomes.