Mouse Brain Function

Mouse brain function encompasses the coordinated neural processes that enable sensation, movement, learning, memory, emotion, and essential physiological regulation. Neurons communicate through electrical impulses and chemical synapses, while interconnected brain regions process sensory information, integrate internal and external signals, and generate adaptive responses. Studying these mechanisms in mice allows researchers to examine neural circuits, behavior, development, and responses to genetic or environmental changes under controlled conditions. Mouse models therefore support investigations of nervous-system biology and disease, including how brain activity contributes to cognition, motor control, and neurological disorders, while providing a foundation for evaluating potential therapeutic strategies.

Mouse Brain Function - Related Videos

Research

JoVE Journal - Biology

Isolation and Functional Analysis of Arteriolar Endothelium of Mouse Brain Parenchyma

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Cited by 2 •

2022

Intensive preparation of intact mouse cerebral endothelial "tubes" from cerebral parenchymal arterioles is illustrated for studying cerebral blood flow regulation. Further, we demonstrate the experimental strengths of this endothelial study model for fluorescence imaging and electrophysiology measurement of key cellular signaling pathways, including changes in intracellular [Ca2+] and membrane potential.

Microdissection of Mouse Brain into Functionally and Anatomically Different Regions

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Cited by 28 •

2021

We present a hands-on, step-by-step, rapid protocol for mouse brain removal and dissection of discrete regions from fresh brain tissue. Obtaining brain regions for molecular analysis has become routine in many neuroscience labs. These brain regions are immediately frozen to obtain high quality transcriptomic data for system level analysis.

Local Application of Drugs to Study Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices

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Cited by 23 •

2012

In this paper, we describe a useful method to study ligand-gated ion channel function in neurons of acutely isolated brain slices. This method involves the use of a drug-filled micropipette for local application of drugs to neurons recorded using standard patch clamp techniques.

Isolating Immune Cells from Mouse Brain and Skull

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Cited by 1 •

2024

To investigate the immune response to brain disorders, one common approach is to analyze changes in immune cells. Here, two simple and effective protocols are provided for isolating immune cells from murine brain tissue and skull bone marrow.

Purification of Mouse Brain Vessels

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Cited by 90 •

2015

We describe a protocol allowing the purification of the mouse brain's vascular compartment. Isolated brain vessels include endothelial cells linked by tight junctions and surrounded by a continuous basal lamina, pericytes, vascular smooth muscle cells, as well as perivascular astroglial membranes.

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