Pollen Source Control
Pollen Source Control - Related Videos
07:07
A High-Resolution, Single-Grain, In Vivo Pollen Hydration Bioassay for Arabidopsis thaliana
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2023
An improved method to measure pollen hydration profiles in Arabidopsis thaliana is described here. The new method offers higher resolution, is noninvasive, and is highly reproducible. The protocol represents a new tool for a finer dissection of the processes that regulate the early stages of pollination.
06:45
Live Imaging of Arabidopsis Pollen Tube Reception and Double Fertilization Using the Semi-In Vitro Cum Septum Method
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Cited by 5 •
2023
Here, we describe an improvement of the semi-in vitro (SIV) method for observing pollen tube guidance and reception in Arabidopsis thaliana, which increases the receptivity of ovules. The high-throughput SIV cum septum method may be coupled with gametophyte marker lines and genetically encoded biosensors to monitor the dynamic process of fertilization.
06:34
A Component-resolved Diagnostic Approach for a Study on Grass Pollen Allergens in Chinese Southerners with Allergic Rhinitis and/or Asthma
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Cited by 4 •
2017
This work describes a protocol that uses a component-resolved approach to study sensitization to grass pollen allergens in a cohort of patients from southern China with allergic rhinitis and/or asthma.
08:11
Collection and Identification of Pollen from Honey Bee Colonies
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Cited by 8 •
2021
We describe methods for collecting corbicular pollen from honey bees as well as protocols for color sorting, acetolysis, and microscope slide preparation of pollen for taxonomic identification. In addition, we present pellet color and taxonomic diversity of corbicular pollen collected from five cropping systems using pollen traps.
10:13
Working with Hot and Cold Sources
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2023
Source: Robert M. Rioux & Suprita Jharimune, Pennsylvania State University, University Park, PA Working with extreme temperatures, both high and low, is an integral part of many laboratory operations. For many, mentioning a laboratory instantly evokes the mental picture of a Bunsen burner. Bunsen burners and hot plates are used extensively in small and large operations in research laboratories and industries, thus making it necessary for all users to be aware of their safe handling...
FAQs
Maintaining a known male parent lets researchers connect the resulting fertilization event to a specific source. This improves confidence when examining inheritance, reproductive compatibility, or traits in breeding material. Without source control, unwanted pollen can introduce an unplanned male parent, making genetic studies, hybrid production, and interpretation of offspring outcomes less reliable.
These approaches provide different ways to reduce the chance that unintended pollen reaches a flower. Floral isolation and bagging restrict opportunities for outside pollen movement, while emasculation and male-sterile plants prevent the flower or plant from contributing unwanted pollen. Selecting among these measures helps preserve the intended parentage of a cross.
Selected pollen must be transferred when the stigma is receptive for the planned fertilization to proceed. This condition links the timing of pollen delivery with the female reproductive surface rather than treating pollen placement as sufficient by itself. Accounting for receptivity therefore helps researchers perform crosses consistently and interpret unsuccessful or successful fertilization more accurately.
A typical sequence begins by identifying the intended male and female plants, reducing opportunities for unwanted pollination, and applying a protective or reproductive control measure. Researchers then transfer the selected pollen to a receptive stigma while maintaining the planned cross. These steps preserve a clear connection between pollen source and fertilization outcome.
The approach is useful whenever the identity of the male parent affects the question being studied. Applications include genetic studies, crop improvement, hybrid seed production, and investigations of pollination, inheritance, or reproductive compatibility. In each case, controlling pollen movement helps distinguish planned biological relationships from effects caused by accidental cross-contamination.
By reducing unintended pollen, the method helps breeders maintain the selected parental combination used to produce experimental or improved material. This is particularly relevant when researchers need offspring associated with a planned cross rather than an unknown pollen source. The resulting control supports more accurate evaluation of inherited characteristics and hybrid outcomes.