Microfluidic Crystallization
Microfluidic Crystallization - Related Videos
08:01
A Microfluidic Approach for the Study of Ice and Clathrate Hydrate Crystallization
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2022
The present protocol describes the crystallization of microscopic ice crystals and clathrate hydrates in microfluidic devices, enabling liquid exchange around the formed crystals. This provides unparalleled possibilities to examine the crystallization process and binding mechanisms of the inhibitors.
10:35
Studies of Bacterial Chemotaxis Using Microfluidics - Interview
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Cited by 1 •
2007
06:19
High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography
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Cited by 14 •
2023
This protocol details high-throughput crystallization screening, ranging from the 1,536 microassay plate preparation to the end of a 6 week experimental time window. Details are included about the sample setup, the imaging obtained, and how users can perform analyses using an artificial intelligence-enabled graphical user interface to quickly and efficiently identify macromolecular crystallization conditions.
09:34
Microfluidic Pneumatic Cages: A Novel Approach for In-chip Crystal Trapping, Manipulation and Controlled Chemical Treatment
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Cited by 6 •
2016
Herein, we describe the fabrication and operation of a double-layer microfluidic system made of polydimethylsiloxane (PDMS). We demonstrate the potential of this device for trapping, directing the coordination pathway of a crystalline molecular material and controlling chemical reactions onto on-chip trapped structures.
07:39
Protein Crystallization
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2023
Protein crystallization, obtaining a solid lattice of biomolecules, elucidates protein structure and enables the study of protein function. Crystallization involves drying purified protein under a combination of many factors, including pH, temperature, ionic strength, and protein concentration. Once crystals are obtained, the protein structure can be elucidated by x-ray diffraction and computation of an electron density model. This video introduces protein crystallization and shows a general...
FAQs
Supersaturation provides the driving condition for both nucleation and subsequent crystal growth, so controlling it is central to microfluidic crystallization. Flow, mixing, temperature, concentration, and solvent composition can be adjusted to regulate how supersaturation develops. This control helps researchers favor reproducible crystal formation and examine how changing conditions affects the resulting crystals.
Small channels and droplets make transport and interfaces especially important. Changes in flow and mixing influence how chemical components encounter one another, while interfaces can affect where crystals form and how they develop. Studying these effects helps explain variations in crystal size, morphology, and composition, rather than treating crystal formation as dependent only on bulk solution conditions.
Temperature, concentration, and solvent composition are distinct control variables, and each can alter the conditions under which crystals appear and grow. Their effects can be examined alongside flow and mixing, allowing researchers to compare different influences on crystallization. The resulting comparisons can identify conditions associated with more uniform crystals or a particular morphology.
Compared with approaches that consume larger sample volumes, microscale crystallization uses small reaction volumes while allowing conditions to be varied systematically. This combination is valuable when sample availability is limited or when many conditions must be tested. The platform supports efficient comparisons of crystallization behavior while maintaining control over the chemical environment.
A typical workflow begins by selecting values for flow, mixing, temperature, concentration, and solvent composition, then introducing the chemical system into channels or droplets. Researchers examine the resulting crystal formation and compare outcomes across conditions. Because the device can support parallel experiments, the workflow can rapidly screen combinations that produce uniform crystals or distinct structural results.
Microfluidic crystallization is useful for screening crystallization conditions before focusing on a preferred set of parameters. Parallel experiments can reveal how changes in chemical conditions alter crystal size, morphology, composition, or growth behavior. In chemistry, this information supports decisions about isolating compounds, investigating crystal growth, and preparing materials with defined structures.
In chemistry, the method connects practical crystal production with questions about structure and transport at small scales. It can be applied to isolate compounds, study how crystals grow, and prepare materials whose structures are intentionally defined. Its reproducible, condition-controlled format also helps investigate how microscale transport and interfaces contribute to final crystal characteristics.