Solid Support
Solid Support - Related Videos
19:56
Introduction to Solid Supported Membrane Based Electrophysiology
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Cited by 35 •
2013
Here we present an electrophysiological method based on solid supported membranes with focus on its applications for the characterization of electrogenic membrane transporters.
10:19
Synthesis and Testing of Supported Pt-Cu Solid Solution Nanoparticle Catalysts for Propane Dehydrogenation
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2017
A convenient method for the synthesis of 2 nm supported bimetallic nanoparticle Pt-Cu catalysts for propane dehydrogenation is reported here. In situ synchrotron X-ray techniques allow for the determination of the catalyst structure, which is typically unobtainable using laboratory instruments.
09:12
Selection of Transporter-Targeted Inhibitory Nanobodies by Solid-Supported-Membrane (SSM)-Based Electrophysiology
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Cited by 3 •
2021
Nanobodies are important tools in structural biology and pose a great potential for the development of therapies. However, the selection of nanobodies with inhibitory properties can be challenging. Here we demonstrate the use of solid-supported-membrane (SSM)-based electrophysiology for the classification of inhibitory and non-inhibitory nanobodies targeting electrogenic membrane transporters.
09:42
Solid Phase Synthesis
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2023
Source: Vy M. Dong and Diane Le, Department of Chemistry, University of California, Irvine, CA Merrifield's solid-phase synthesis is a Nobel Prize winning invention where a reactant molecule is bound on a solid support and undergoes successive chemical reactions to form a desired compound. When the molecules are bound to a solid support, excess reagents and byproducts can be removed by washing away the impurities, while the target compound remains bound to the resin. Specifically, we will...
02:37
Metallic Solids
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2020
Metallic solids such as crystals of copper, aluminum, and iron are formed by metal atoms. The structure of metallic crystals is often described as a uniform distribution of atomic nuclei within a “sea” of delocalized electrons. The atoms within such a metallic solid are held together by a unique force known as metallic bonding that gives rise to many useful and varied bulk properties. All metallic solids exhibit high thermal and electrical conductivity, metallic luster, and malleability. Many...
FAQs
The linker determines how a bound molecule is handled during synthesis: it attaches the starting compound to the support and can later be cleaved to release the product. This separation between attachment and release allows reagents to act on the immobilized intermediate through successive reaction cycles, while preserving a route to recover the synthesized material.
Repeated washing is central to the method because excess reagents and other materials remain in the reaction mixture after each transformation. Filtration then separates the insoluble support from that mixture. Together, these operations reduce the need to isolate the bound intermediate after every cycle, which is why solid support can simplify purification and reaction handling.
Compared with a sequence in which intermediates remain in solution, a solid-support strategy keeps the growing or reacting molecule associated with an insoluble phase during processing. That physical arrangement enables reaction, washing, and separation to be repeated in a cycle. Its value is therefore not only chemical transformation, but also a more manageable workflow for multistep preparation.
A typical supported-synthesis cycle begins with a starting compound attached through a cleavable linker. Reagents are added to react with the bound molecule, excess materials are removed by washing, and filtration separates the support from the reaction mixture. Repeating this sequence builds the desired preparation before cleavage releases material from the support.
The core setup includes an insoluble support, often polymer beads, a cleavable linker, the starting compound, and reagents for each reaction. Washing and filtration are essential handling steps, while the support's physical form permits repeated processing and can support automated preparation. These components together make successive reactions easier to manage than repeated intermediate isolation.
Solid support is especially useful in peptide and oligonucleotide synthesis, where repeated reaction cycles are important. It also supports combinatorial chemistry, which can generate diverse chemical libraries, and supported catalysis, where catalysts remain associated with an insoluble material during reactions. These uses connect the method to both synthesis and reaction handling.