Jacob Staff
Jacob Staff - Related Videos
08:33
High-resolution Respirometry to Assess Mitochondrial Function in Permeabilized and Intact Cells
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Cited by 69 •
2017
High-resolution respirometry is used to determine mitochondrial oxygen consumption. This is a straightforward technique to determine mitochondrial respiratory chain complexes' (I-IV) respiratory rates, maximal mitochondrial electron transport system capacity, and mitochondrial outer membrane integrity.
10:25
Isolation of Intact Mitochondria from Skeletal Muscle by Differential Centrifugation for High-resolution Respirometry Measurements
0 Views •
Cited by 26 •
2017
Here, a quadriceps muscle specimen is taken from an anaesthetized pig and mitochondria are isolated by differential centrifugation. Then, the respiratory rates of mitochondrial respiratory chain complexes I, II and IV are determined using high-resolution respirometry.
08:32
Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo
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2016
This study describes biophysical, biochemical and molecular techniques to characterize the chaperone activity of Escherichia coli HdeB under acidic pH conditions. These methods have been successfully applied for other acid-protective chaperones such as HdeA and can be modified to work for other chaperones and stress conditions.
10:16
SorLA and CLC:CLF-1-dependent Downregulation of CNTFRα as Demonstrated by Western Blotting, Inhibition of Lysosomal Enzymes, and Immunocytochemistry
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Cited by 1 •
2017
The present protocol describes how Western blotting and immunocytochemistry combined with inhibition of lysosomal enzymes can be used to demonstrate the downregulation of Ciliary Neurotrophic Factor Receptor-α (CNTFRα), which is conveyed by the interaction between Cytokine-like Factor-1 (CLF-1) and the endocytic receptor sorLA.
09:36
Safety Precautions and Operating Procedures in an (A)BSL-4 Laboratory: 4. Medical Imaging Procedures
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Cited by 8 •
2016
Here, we present an overview of the preparation and animal handling procedures required to safely perform medical imaging in an animal biosafety level 4 laboratory. Computed tomography of a mock-infected guinea pig illustrates these procedures that may be used to evaluate the disease caused by a high consequence pathogen.
FAQs
The instrument relies on the geometric relationship between the position of the sliding crosspiece and the two lines of sight. One sighting edge is aligned with a reference point and another with the target, creating a measurable angular relationship. Reading the crosspiece position on the graduated rod then provides the basis for estimating relative direction, height, or distance.
The graduated rod provides a scale for recording the crosspiece position, while the crosspiece establishes the two sighting directions needed for comparison. Their interaction turns a visual alignment into a repeatable reading rather than an informal estimate. Consistent alignment of the sighting edges is therefore essential for obtaining a meaningful geometric result in field surveying.
Alignment determines whether the reference point and target correspond correctly to the instrument's geometric reading. If a sighting edge is not directed toward the intended point, the recorded crosspiece position represents a different angular relationship and can affect estimated distance, height, or relative position. Careful visual alignment is consequently central to reliable field measurements.
Jacob Staff uses direct visual alignment and geometric reading, so it demonstrates measurement principles without relying on electronic instrumentation. Modern electronic instruments provide greater precision, making them more suitable when highly accurate results are required. The older device remains useful where the goal is to understand basic surveying geometry, practice field observation, or illustrate how measurements relate to angles.
A user positions the staff, identifies a reference point and a target, and aligns the instrument's sighting edges with both points. The sliding crosspiece is then adjusted to establish the required visual relationship, and its position is read from the graduated rod. That reading is interpreted through geometric relationships to estimate the desired field quantity.
In engineering and surveying instruction, the instrument connects observable field alignments with geometric measurement. Students can use it to explore how angular relationships support estimates of distance, height, and relative position, while also examining the practical limits of non-electronic tools. It also provides historical context for topographic mapping, land measurement, and basic astronomical or navigational observations.