Ponding Method

The ponding method is an engineering technique for measuring how quickly water infiltrates a soil or porous surface, providing information for drainage, irrigation, and stormwater design. In a typical test, water is maintained as a shallow pond over a defined area, and the volume or water-level change required to sustain the pond is recorded over time; the resulting infiltration rate reflects the surface’s hydraulic conductivity under the test conditions. Engineers use these measurements to evaluate soil permeability, estimate runoff and groundwater recharge, assess infiltration facilities, and determine whether site soils can support systems such as recharge basins, permeable pavements, or septic leach fields.

Ponding Method - Related Videos

Research

JoVE EoE - Neuropathology

Inducing Massive Pontine Hemorrhage in a Rat Model by Autologous Blood Injection

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2025

Source: Tang, X. et. al., Massive Pontine Hemorrhage by Dual Injection of Autologous Blood. J. Vis. Exp. (2021)This protocol describes the controlled infusion of autologous blood into the pontine region of a rat's brain to model massive pontine hemorrhage. The purpose is to simulate a localized brain bleed by increasing localized pressure.

Isolating and Culturing Cells from Diffuse Intrinsic Pontine Glioma

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2025

This video demonstrates the process of isolating and culturing glial progenitor cells from diffuse intrinsic pontine glioma, or DIPG-affected brainstem tissue. Glial cells isolated from tumor tissue via an enzymatic digestion are cultured in a neurobasal medium, which promotes their growth and the development of free-floating neurospheres.

Research

JoVE Journal - Cancer Research
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A Protocol for Rapid Post-mortem Cell Culture of Diffuse Intrinsic Pontine Glioma (DIPG)

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Cited by 55 •

2017

This protocol describes a method for the rapid processing of post-mortem diffuse intrinsic pontine glioma samples for the establishment of patient-derived cell culture models or direct characterization of tumor and microenvironmental cells.

Establishment of Acute Pontine Infarction in Rats by Electrical Stimulation

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Cited by 7 •

2020

Presented here is a protocol for establishing acute pontine infarction in a rat model via electrical stimulation with a single pulse.

Massive Pontine Hemorrhage by Dual Injection of Autologous Blood

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Cited by 3 •

2021

We present a protocol to establish a massive pontine hemorrhage model in a rat via dual injection of autologous blood.

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