Bioactive Molecule Encapsulation

Bioactive molecule encapsulation is the incorporation of biologically active compounds within protective carriers to preserve their function and regulate their delivery. Encapsulation can use lipid vesicles, polymeric particles, or other materials that shield molecules from degradation and release them through diffusion, carrier breakdown, or environmental triggers. In genetics, this approach supports the delivery of nucleic acids, proteins, and regulatory compounds into cells, where controlled exposure can influence gene expression and cellular responses. It therefore enables gene-function studies, improves the stability and targeting of molecular tools, and contributes to the development of delivery systems for genetic research and emerging therapies.

Bioactive Molecule Encapsulation - Related Videos

Research

JoVE Journal - Bioengineering
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Encapsulating Cytochrome c in Silica Aerogel Nanoarchitectures without Metal Nanoparticles while Retaining Gas-phase Bioactivity

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2016

This procedure describes how to encapsulate cytochrome c (cyt. c) in silica (SiO2) sol-gels, process these gels to form bioaerogels, and use these bioaerogels to rapidly recognize nitric oxide (NO) through a gas-phase reaction. This type of protocol may aid in the future development of biosensors or other bioanalytical devices.

Research

JoVE Journal - Biochemistry
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Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy

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2024

This article describes the dual-color labeling of long RNAs at termini positions and their surface immobilization via encapsulation in phospholipid vesicles for single-molecule FRET TIRF microscopy applications. Combining these techniques enables precise visualization and analysis of RNA dynamics at the single-molecule level.

Research

JoVE Journal - Biochemistry

Formulation and Characterization of Bioactive Agent Containing Nanodisks

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2023

Here, we describe the production and characterization of bioactive agents containing nanodisks. Amphotericin B nanodisks are taken as an example to describe the protocol in a stepwise manner.

Encapsulating Nerve Growth Factor in High-Density Lipoprotein-Mimicking Nanoparticles

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2025

Source: Zhu, J., et. al., Preparation and Characterization of Novel HDL-mimicking Nanoparticles for Nerve Growth Factor Encapsulation. J. Vis. Exp. (2017)This video demonstrates the stepwise assembly of NGF-loaded HDL-mimicking nanoparticles using protamine-assisted encapsulation and lipid-based self-assembly for potential neurotrophic delivery applications.

Quantification of Violacein in Chromobacterium violaceum and Its Inhibition by Bioactive Compounds

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2025

The objective of this protocol is to quantify violacein production by Chromobacterium violaceum ATCC 12472 as a proxy for assessing quorum sensing inhibition by bioactive compounds.

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