Relative Thickness Quantification

Relative thickness quantification is the measurement of a layer’s thickness as a ratio to a reference thickness or characteristic length, providing a dimensionless way to compare samples and structures. It typically involves measuring the target thickness with a calibrated technique, selecting a consistent reference, and calculating the ratio, so differences in scale or experimental setup can be evaluated more reliably. In physics, this approach supports the characterization of thin films, coatings, membranes, and layered materials, where thickness influences optical, thermal, electrical, and mechanical behavior. Comparing relative values helps researchers identify uniformity, assess fabrication quality, and relate structure to observed physical properties.

Relative Thickness Quantification - Related Videos

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JoVE Journal - Bioengineering
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Localization and Relative Quantification of Carbon Nanotubes in Cells with Multispectral Imaging Flow Cytometry

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Cited by 19 •

2013

In this study, the main purpose was to monitor the cellular uptake and eventual exocytosis of carbon nanotubes (CNTs). From this perspective, we proposed here a unique method using multispectral imaging flow cytometry allowing a quantification and localization of CNTs in a statistically relevant number of cells.

Research

JoVE Journal - Engineering

Quantifying the Relative Thickness of Conductive Ferromagnetic Materials Using Detector Coil-Based Pulsed Eddy Current Sensors

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Cited by 10 •

2020

Here, we present a protocol to quantify the relative thickness (i.e., thickness as a percentage with respect to a reference) of conductive ferromagnetic materials using detector coil-based pulsed eddy current sensors, while overcoming the calibration requirement.

Murine Full-thickness Skin Transplantation

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Cited by 45 •

2017

Murine full-thickness skin transplantation is a well-established model to study rejection in an alloimmune setting. Here, we provide a tutorial of each step involved in performing a BALB/c-->C57BL/6 full-thickness skin transplant.

Research

JoVE Journal - Medicine
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Measuring Diaphragm Thickness and Function Using Point-of-Care Ultrasound

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Cited by 9 •

2023

Diaphragm thickness and function can be assessed in healthy individuals and critically ill patients using point-of-care ultrasound. This technique offers an accurate, reproducible, feasible, and well-tolerated method for evaluating diaphragm structure and function.

Organotypic Culture of Full-thickness Adult Porcine Retina

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Cited by 19 •

2011

Here we describe a cost-effective technique for organotypic culture of adult porcine retina for seven days. Briefly, a sterile filter paper was used to lift the neural retina off from the RPE and place photoreceptor side up on an insert raised by a custom-made stand.

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