Command Following
Command Following - Related Videos
05:00
Rapid Characterization of Genetic Parts with Cell-Free Systems
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Cited by 5 •
2021
Well-characterized genetic parts are necessary for the design of novel genetic circuits. Here we describe a cost-effective, high-throughput method for rapidly characterizing genetic parts. Our method reduces cost and time by combining cell-free lysates, linear DNA to avoid cloning, and acoustic liquid handling to increase throughput and reduce reaction volumes.
12:05
Preparation of Hydrophobic Metal-Organic Frameworks via Plasma Enhanced Chemical Vapor Deposition of Perfluoroalkanes for the Removal of Ammonia
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Cited by 16 •
2013
Herein the procedures for plasma enhanced chemical vapor deposition of perfluoroalkanes on microporous materials such as metal-organic frameworks to enhance their stability and hydrophobicity are described. Furthermore, breakthrough testing of milligram quantities of samples is described in detail.
10:46
A Method of Trigonometric Modelling of Seasonal Variation Demonstrated with Multiple Sclerosis Relapse Data
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Cited by 2 •
2015
Combining plot analysis with trigonometric regression is a robust method for exploring complex, cyclical phenomena such as relapse onset timing in multiple sclerosis (MS). This method enabled unbiased characterisation of seasonal trends in relapse onset permitting novel inferences around the influence of seasonal variation, ultraviolet radiation (UVR) and latitude.
06:02
A Computational Pipeline for Intergenic/Intragenic Enhancer RNA Quantification in Mouse Embryonic Stem Cells
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2025
This protocol provides a streamlined computational pipeline for quantifying nascent enhancer transcripts. By integrating chromatin accessibility, chromatin feature, and transcriptional data, it enables accurate detection and strand-specific analysis of enhancer activity in complex intragenic regions, while remaining accessible to researchers without extensive bioinformatics training.
06:25
Using Computer Vision Libraries to Streamline Nuclei Quantification
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Cited by 1 •
2025
This article describes step-by-step methods to automate image-based nuclei quantification using an open-source executable program validated across a range of cell densities. This program provides an alternative that addresses barriers related to cost, accessibility for users with limited technological skillsets, and application-specific validation that may limit utility of existing technologies.
FAQs
A cue becomes effective when it reliably predicts a desired outcome. Conditioning links the cue with that outcome, while reinforcement strengthens the likelihood that the individual will perform the expected action again. In behavioral research, changes in response accuracy and timing can therefore indicate how strongly learning has established the cue-action relationship.
Stimulus discrimination allows an individual to respond selectively to the relevant verbal, visual, or physical cue rather than reacting to every signal. Researchers can examine whether responses remain accurate when cues differ, which helps distinguish precise learning from generalized responding and provides evidence about attention and behavioral control.
Attention, learning history, memory, communication, and behavioral flexibility can all shape performance. A response may become more accurate as the cue-outcome relationship strengthens, while memory supports retaining the learned association. Measuring both whether the action occurs and how quickly it occurs helps reveal differences in these underlying behavioral processes.
Researchers can present a specified cue and record whether the expected action occurs, how accurately it matches the cue, and how promptly it is performed. Repeated measurement during acquisition shows how the response develops, whereas later assessment of performance shows whether the learned behavior is maintained over time.
Because it produces an observable response to a defined cue, command following can provide a measurable assessment of attention, learning, memory, communication, and behavioral flexibility. Researchers and practitioners can apply it in animal training, education, and clinical assessment, selecting the relevant performance measure according to the ability or behavior under study.
The same broad behavioral framework can be examined across humans and other animals by comparing responses to verbal, visual, or physical cues and tracking accuracy or timing. This supports research on how learning is shaped by environmental conditions and provides context for investigating neural and environmental factors that influence behavior.