Cell Barrier Function
Cell Barrier Function - Related Videos
12:30
Electric Cell-substrate Impedance Sensing for the Quantification of Endothelial Proliferation, Barrier Function, and Motility
0 Views •
Cited by 213 •
2014
This protocol reviews Electric Cell-substrate Impedance Sensing, a method to record and analyze the impedance spectrum of adherent cells for the quantification of cell attachment, proliferation, motility, and cellular responses to pharmacological and toxic stimuli. Detection of endothelial permeability and assessment of cell-cell and cell-substrate contacts are emphasized.
09:21
A Triple Culture Cell System Modeling the Human Blood-Brain Barrier
0 Views •
Cited by 11 •
2021
This protocol describes a method to establish a human blood-brain barrier (BBB) in vitro model. The endothelial cells and pericytes are seeded on each side of an insert filter (blood compartment), and astrocytes are seeded in the bottom well (brain compartment). The model characterized was used for nanoparticles transport experiments.
09:03
Barrier Functional Integrity Recording on bEnd.3 Vascular Endothelial Cells via Transendothelial Electrical Resistance Detection
0 Views •
Cited by 4 •
2023
This protocol describes a dependable and efficient in vitro model of the brain blood barrier. The method uses mouse cerebral vascular endothelial cells bEnd.3 and measures transmembrane electrical resistance.
09:10
Use of the MicroSiM (µSiM) Barrier Tissue Platform for Modeling the Blood-Brain Barrier
0 Views •
Cited by 8 •
2024
This report provides protocols for assembly, cell culture, and assays on the µSiM platform for the construction of blood-brain barrier models.
02:04
Generation of a Human Colonoid Monolayer with Barrier Function to Study Host–Pathogen Interactions
0 Views •
2026
Source: In, J. G.,et al. Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium. J. Vis. Exp. (2019).This video demonstrates the procedure for culturing colon-derived epithelial cells on a collagen-coated Transwell system to generate a mucus-producing monolayer that models the intestinal barrier and enables host–pathogen interaction studies.
FAQs
Passage across a cell barrier reflects the combined action of selective permeability, cell-cell junctions, and membrane transporters rather than a single gate. Junctions help maintain the continuity of the cell layer, while transporters contribute to regulated substance movement across cell membranes. Considering these features together allows a model to represent both tissue integrity and controlled exchange between compartments.
Size, charge, and solubility are key properties identified for regulated passage across epithelial and endothelial layers. Evaluating these variables helps researchers interpret why different substances may cross a model differently. In bioengineering studies, they provide a basis for examining selective permeability and for relating transport behavior to the biological compartment being represented.
Maintaining tissue integrity matters because a model can only represent regulated exchange if its cellular layer remains organized enough to separate compartments. In bioengineering, this makes junctional organization an important consideration alongside transport measurements. A barrier model with compromised integrity may not reflect the selective behavior of epithelial or endothelial tissues used to represent organs.
Researchers combine cultured cells with biomaterials and, in some systems, microfluidic platforms to model barrier-relevant tissue arrangements. The cultured cells provide the biological layer, while the engineered components help create an experimental system for controlled exchange between compartments. This combination can be adapted to study barriers associated with different organs.
Microfluidic platforms are used as engineered settings for modeling epithelial and endothelial barriers. They can be combined with cultured cells and biomaterials to create a more physiologically relevant experimental system. Such systems support investigation of transport, disease, drug delivery, and toxicity within a barrier-focused model, extending analysis beyond conventional cell culture formats.
Bioengineered models can represent barriers associated with the intestine, kidney, lung, and blood-brain interface. This range connects barrier studies with organ-specific questions about transport and tissue behavior. By selecting a relevant cellular and engineered context, researchers can investigate how barrier properties relate to disease, drug delivery, toxicity, or other processes in particular biological compartments.
These systems are useful when researchers seek a more physiologically relevant way to study transport, disease, drug delivery, or toxicity. Combining cultured cells with biomaterials and microfluidic platforms helps develop alternatives to conventional cell cultures and animal models. Their purpose is not merely to reproduce a cell layer, but to create experimental contexts that better represent tissue-associated barrier behavior.