Led Construction

LED construction is the design and assembly of light-emitting diodes, semiconductor devices that convert electrical energy into visible or near-infrared light. In a typical LED, a p-n junction combines differently doped semiconductor layers; when forward-biased, electrons and holes recombine in the active region and release photons, while electrodes, substrates, encapsulants, and lenses support electrical connection, heat dissipation, and light delivery. In bioengineering, engineered LEDs provide controlled illumination for optical sensing, microscopy, photobiomodulation, and studies of cell or tissue responses to light. Their tunable wavelength, compact size, and low energy demand support portable instruments and precisely controlled biological experiments.

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Research

JoVE Journal - Neuroscience

Using Affordable LED Arrays for Photo-Stimulation of Neurons

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Cited by 7 •

2011

Adult-born neurons expressing ChR2 can be manipulated in slice electrophysiological preparations in order to examine their contribution towards the function of olfactory neural circuits.

Estimating the Yield of Compounds on the TLC Plate via the Blue-LED Illumination Technique

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Cited by 1 •

2022

The present protocol developed a method to estimate the yield of compounds on the TLC plate using the blue-LED illumination technique. The advantages of this approach are that it is safe, effective, inexpensive, and allows the researcher to measure multiple samples simultaneously.

Research

JoVE Journal - Biochemistry

LED-Based In Vitro Screening for Assessing Photoactivable Molecules in Bacterial Photodynamic Inactivation

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2025

This protocol describes a straightforward and economical method for evaluating the effectiveness of potential photosensitizers in antibacterial photodynamic inactivation (aPDI), using a 96-well plate format combined with an LED panel light source. This approach enables the simultaneous testing of multiple experimental conditions, including different concentrations, compounds, and bacterial strains.

Research

JoVE Journal - Cancer Research
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Development of an Innovative LED-based Illumination Device for In Vitro Application of Photodynamic Therapy with Rose Bengal

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2025

This manuscript presents a novel, innovative 3D-printed illumination device for studying Rose Bengal-mediated photodynamic therapy in vitro.

LED Thermo Flow — Combining Optogenetics with Flow Cytometry

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Cited by 5 •

2016

Optically controlled substances are powerful tools to study signaling pathways. To expand the spectrum of possible experiments, we developed a device for studying optically controlled substances in real time using flow cytometry: the LED Thermo Flow.

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