Mechanical Analysis
Mechanical Analysis - Related Videos
08:42
Myo-mechanical Analysis of Isolated Skeletal Muscle
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Cited by 25 •
2011
To assess the in vivo effects of therapeutic interventions for muscle disease, methods are needed to quantitate force generation and fatigability in treated muscle. We detail an approach to evaluating myo-mechanical properties in explanted mouse hindlimb muscle. This analysis provides a robust approach to quantitating the effects of genetic modification on muscle function, as well as comparison of therapies in mouse models of muscle disease.
05:35
Ex Vivo Analysis of Mechanically Activated Ca2+ Transients in Urothelial Cells
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2022
This protocol describes a methodology to assess the function of mechanically activated ion channels in native urothelial cells using the fluorescent Ca2+ sensor GCaMP5G.
12:45
Mechanical Stimulation of Chondrocyte-agarose Hydrogels
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Cited by 29 •
2012
The biosynthesis of cartilaginous extracellular matrix by chondrocytes can be affected by application of mechanical stimuli. This method describes the technique of applying dynamic compressive strains to chondrocytes encapsulated in 3D constructs and the evaluation of induced changes in chondrocyte metabolism.
05:06
Mechanical Dissociation of Tissues for Single Cell Analysis Using a Motorized Device
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Cited by 3 •
2023
A general protocol for the combined enzymatic and semi-automated mechanical dissociation of tissues to generate single-cell suspensions for downstream analyses, such as flow cytometry, is provided. Instructions for the fabrication, assembly, and operation of the low-cost mechanical device developed for this protocol are included.
08:43
Imaging of the Microstructural Failure Mechanism in the Human Hip
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Cited by 1 •
2023
The protocol enables the measurement of the deformation of the bone microstructure in the entire proximal human femur and its toughness by combining large-volume micro-CT scanning, a custom-made compressive stage, and advanced image processing tools.
FAQs
Stress and strain provide paired measurements for relating an applied load to the resulting deformation. Examining their relationship helps characterize whether a tissue, biomaterial, or engineered construct resists shape change under loading. These measurements also supply quantitative inputs for mechanical models, allowing researchers to compare materials or structures and connect mechanical behavior with biological function.
Stiffness indicates how strongly a material or structure resists deformation, while elasticity describes deformation behavior associated with changes in shape. Viscoelasticity captures additional mechanical behavior that cannot be represented by a purely elastic response. Considering these measures together gives a more complete description of tissues, cells, biomaterials, and constructs than relying on a single mechanical property.
Mechanical responses can depend on the conditions under which a biological system is examined. Testing tissues, cells, implants, or engineered constructs in conditions that approximate their physiological environment makes the measurements more relevant to intended biological use. This context supports better interpretation of structure-function relationships and improves assessment of compatibility, safety, and expected performance.
A controlled experiment applies defined forces or motions and records the resulting deformation or functional change. Researchers can then use mechanical models to organize those measurements and relate loading conditions to observed behavior. Combining experimental data with modeling supports quantitative characterization, comparison among materials or biological systems, and interpretation of how structure contributes to mechanical function.
A typical workflow identifies the biological material, structure, or system being studied, selects relevant force or motion conditions, and measures the resulting deformation or response. Investigators then evaluate properties such as stress, strain, stiffness, elasticity, or viscoelasticity and interpret the results with mechanical models. The findings can guide design decisions or evaluate performance.
Mechanical data help determine whether a medical device or tissue scaffold can provide the intended structural response under relevant loading conditions. Measurements can guide material and structural design, while testing supports evaluation of safety, durability, and compatibility. In tissue engineering, the results also help relate scaffold behavior to biological function and assess engineered constructs quantitatively.