Paramagnetic Reagent
Paramagnetic Reagent - Related Videos
11:07
Electron Paramagnetic Resonance (EPR) Spectroscopy
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2023
Source: David C. Powers, Tamara M. Powers, Texas A&M In this video, we will learn the basic principles behind Electron Paramagnetic Resonance (EPR). We will use EPR spectroscopy to study how dibutylhydroxy toluene (BHT) behaves as an antioxidant in the autoxidation of aliphatic aldehydes.
01:30
Paramagnetism
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2023
Paramagnets are materials with unpaired electrons that possess a finite magnetic moment. In the absence of a magnetic field, these moments are randomly oriented, and thus the net moment is zero. Under an external field, a torque acting on the moments tends to align them along the field's direction. However, the random thermal motion of electrons produces a torque opposite to the external field and tries to disorient the moments. These two competing effects align only a few moments along the...
10:17
Preparing Anhydrous Reagents and Equipment
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2023
Source: Laboratory of Dr. Dana Lashley - College of William and Mary Demonstrated by: Timothy Beck and Lucas Arney Many reactions in organic chemistry are moisture-sensitive and must be carried out under careful exclusion of water. In these cases the reagents have a high affinity to react with water from the atmosphere and if left exposed the desired reaction will not take place or give poor yields, because the reactants are chemically altered. In order to prevent undesired reactions with H2O...
07:24
Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins
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Cited by 2 •
2021
A protocol for the application of paramagnetic relaxation enhancement NMR spectroscopy to detect weak and transient inter- and intra-molecular interactions in intrinsically disordered proteins is presented.
07:07
Tumor Hypoxia Assessment: In Vivo 3D Oxygen Imaging Through Electron Paramagnetic Resonance
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Cited by 1 •
2025
Quantitative 3D oxygen maps of murine tumors were imaged noninvasively using Pulse Electron Paramagnetic Resonance. Ultrasound B-mode and Power Doppler were used for anatomy and vascular structure. Images from both modalities were superimposed enabling multi-parametric tumor analysis.
FAQs
Unpaired electrons create the magnetic response that allows the reagent to be attracted toward an external field. This attraction provides a controllable way to direct reagent-associated material during handling or detection. Because the response depends on the applied field, researchers can use magnetic control to influence where labeled cells, proteins, nucleic acids, or other targets are collected.
Attached ligands provide selectivity by binding particular biological targets, including cells, proteins, nucleic acids, or other molecules. The magnetic component supplies field responsiveness, while the ligand determines which material is recruited or detected. Combining these functions lets bioengineers adapt a reagent to a specific purification, isolation, or analytical task without relying on magnetic behavior alone.
Minimal retained magnetization allows the reagent-associated material to respond primarily when a magnetic field is applied. This supports controlled manipulation and helps release or redistribute material when field guidance is no longer needed. In bioengineering workflows, that behavior is relevant when samples must be directed during one stage but remain manageable for subsequent processing or analysis.
Target recognition and magnetic movement serve different roles. Functionalized ligands determine whether the reagent associates with a desired cell, protein, nucleic acid, or other target, whereas the paramagnetic component enables field-directed handling. This separation of functions helps connect molecular selectivity with physical control, supporting applications in which specific biological material must be isolated or measured.
A conceptual workflow begins by allowing functionalized reagent components to bind the intended targets, followed by applying an external magnetic field to direct the associated material. The selected fraction can then support sample purification or cell isolation, while the field provides the means of controlling material location. The exact workflow depends on the target and assay purpose.
Researchers may choose these reagents when a biological sample requires selective magnetic handling or when molecular interactions need analytical detection. Supported uses include sample purification, cell isolation, biosensing, contrast-enhanced imaging, and assays involving proteins or nucleic acids. The approach is especially useful when ligand-mediated recognition and field-responsive control need to operate together.
In biosensing, magnetic properties can help reveal biological structures or molecular interactions associated with the reagent and its bound target. In contrast-enhanced imaging, the same field-responsive characteristics contribute to making biological structures more detectable. These applications extend the reagent beyond physical separation, using its magnetic behavior as part of an analytical or visualization strategy.