High-throughput Yeast Array
High-throughput Yeast Array - Related Videos
08:57
High-throughput Yeast Plasmid Overexpression Screen
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Cited by 12 •
2011
Here we describe a plasmid overexpression screen in Saccharomyces cerevisiae, using an arrayed plasmid library and a high-throughput yeast transformation protocol with a liquid handling robot.
03:26
High-Throughput Optogenetics Experiments in Yeast Using the Automated Platform Lustro
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Cited by 3 •
2023
This protocol outlines the steps for utilizing the automated platform Lustro to perform high-throughput characterization of optogenetic systems in yeast.
06:27
The Serial Anesthesia Array for the High-Throughput Investigation of Volatile Agents Using Drosophila melanogaster
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Cited by 2 •
2023
The fruit fly (Drosophila melanogaster) is widely used for biological and toxicological research. To expand the utility of flies, we developed an instrument, the serial anesthesia array, that simultaneously exposes multiple fly samples to volatile general anesthetics (VGAs), making it possible to investigate the collateral effects (toxic and protective) of VGAs.
10:02
Applications of pHluorin for Quantitative, Kinetic and High-throughput Analysis of Endocytosis in Budding Yeast
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Cited by 18 •
2016
Accurate quantification of vesicular trafficking events often provides key insights into roles for specific proteins and the effects of mutations. This paper presents methods for using superecliptic pHluorin, a pH-sensitive GFP variant, as a tool for quantification of endocytic events in living cells using quantitative fluorescence microscopy and flow cytometry.
05:04
Active Probe Atomic Force Microscopy with Quattro-Parallel Cantilever Arrays for High-Throughput Large-Scale Sample Inspection
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Cited by 5 •
2023
Large-scale sample inspection with nanoscale resolution has a wide range of applications, especially for nanofabricated semiconductor wafers. Atomic force microscopes can be a great tool for this purpose, but are limited by their imaging speed. This work utilizes parallel active cantilever arrays in AFMs to enable high-throughput and large-scale inspections.
FAQs
Their organized layout places many yeast strains in a format that allows the same environmental or chemical condition to be assessed across different genetic backgrounds. Comparing growth, survival, or another measured phenotype helps reveal strain-specific responses. Those response patterns can then connect differences in phenotype with particular genes, supporting genome-wide genetic screens and gene-function studies.
Robotic pinning transfers small amounts of each yeast strain or colony onto defined media in a controlled, repeatable pattern. This automated handling makes it practical to test many strains in parallel while maintaining consistent placement and treatment conditions. The resulting organization supports reproducible comparisons of growth, survival, or other phenotypes across the array.
Defined media and chemical treatments establish the conditions under which yeast phenotypes are compared. Changing those conditions can expose differences in growth or survival among strains, helping researchers associate environmental or chemical responses with genetic backgrounds. This approach supports drug sensitivity testing and investigations of cellular pathways by showing which strains respond differently under a selected condition.
A phenotype pattern becomes informative when responses are compared across strains with different genetic backgrounds. If particular strains show altered growth, survival, or treatment sensitivity, the associated genetic differences provide clues about gene function. Linking these observations to genes allows the array to support genome-wide screens and helps identify genes involved in cellular pathways.
A typical workflow begins by organizing yeast strains or colonies, then using robotic pinning or automated handling to place small amounts onto defined media. Researchers expose the arranged strains to environmental or chemical conditions and measure growth, survival, or another phenotype. They compare the resulting responses across strains to connect observed differences with genetic backgrounds or genes.
These arrays are useful when researchers need to examine many genetic backgrounds or conditions efficiently. Applications include genome-wide genetic screens, gene-function studies, drug sensitivity testing, and analysis of cellular pathways. Because yeast responses can help identify conserved biological mechanisms and candidate targets for further study, the approach connects large-scale screening with broader questions in biology.