Humidity Aging Protocol

A humidity aging protocol is a controlled method for evaluating how moisture affects the stability, performance, and chemical properties of a material or product over time. In this approach, samples are exposed to defined relative humidity and temperature conditions for a specified period, then examined for changes such as degradation, physical damage, altered composition, or loss of function. In chemistry, humidity aging supports stability testing, formulation development, packaging assessment, and quality control by revealing moisture sensitivity and potential failure pathways. Consistent sample preparation, environmental monitoring, exposure duration, and post-aging analysis improve reproducibility and help predict storage behavior.

Humidity Aging Protocol - Related Videos

Research

JoVE Journal - Engineering
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Nanostructured Ag-zeolite Composites as Luminescence-based Humidity Sensors

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Cited by 9 •

2016

A protocol for the synthesis of moisture-responsive luminescent Ag-zeolite composites is described in this report.

Education

JoVE Science Education - Advanced Biology

An Introduction to Aging and Regeneration

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2023

Tissues are maintained through a balance of cellular aging and regeneration. Aging refers to the gradual loss of cellular function, and regeneration is the repair of damaged tissue generally mediated by preexisting adult or somatic stem cells. Scientists are interested in understanding the biological mechanisms behind these two complex processes. By doing so, researchers may be able to use somatic stem cells to treat degenerative diseases and develop therapies that could delay the effects of...

Research

JoVE Journal - Genetics

A Protocol to Evaluate and Quantify Retinal Pigmented Epithelium Pathologies in Mouse Models of Age-Related Macular Degeneration

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Cited by 1 •

2023

Mouse models can be useful tools for investigating the biology of the retinal pigmented epithelium (RPE). It has been established that mice can develop an array of RPE pathologies. Here, we describe a phenotyping protocol to elucidate and quantify RPE pathologies in mice using light, transmission electron, and confocal microscopy.

Continuous High-resolution Microscopic Observation of Replicative Aging in Budding Yeast

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Cited by 10 •

2013

We describe here the operation of a microfluidic device that allows continuous and high-resolution microscopic imaging of single budding yeast cells during their complete replicative and/or chronological lifespan.

Measuring Single-Cell Aging with an Imaging-based Biomarker of Chromatin and Epigenetic Aging

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2026

The protocol presents the imaging and computational workflow to extract and validate imaging-based chromatin and epigenetic age (ImAge).

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