Eyeball Segmentation

Eyeball segmentation is an image-analysis technique that separates the eyeball and its anatomical components from surrounding tissue or background in biomedical images. It works by identifying boundaries through differences in intensity, color, texture, or shape, often using thresholding, edge detection, deformable models, or machine-learning algorithms to generate a pixel-level mask. In engineering, these masks support three-dimensional reconstruction, biometric identification, ophthalmic measurement, and computer-assisted diagnosis. Accurate segmentation enables researchers to quantify ocular structures, track changes across images, and develop reliable systems for eye-tracking, surgical planning, and computational modeling.

Eyeball Segmentation - Related Videos

Education

JoVE Core - Anatomy and Physiology

Anatomy of the Eyeball

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2024

The eye is a spherical, hollow structure composed of three tissue layers. The outer layer — the fibrous tunic, comprises the sclera — a white structure — and the cornea, which is transparent. The sclera encompasses some of the ocular surface, most of which is not visible. However, the 'white of the eye' is distinctively visible in humans compared to other species. The cornea, a clear covering at the front of the eye, enables light penetration. The eye's middle layer, the vascular tunic,...

Research

JoVE Journal - Medicine

Preparation and Analysis of Histological Slides of Rat and Mouse Eyeballs to Evaluate the Retina

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Cited by 1 •

2024

This manuscript presents a protocol for the histological preparation of slides from rodent eyeballs. With the proposed method, the inner retina can be easily visualized and assessed under a light microscope. The procedure is presented for the eyeballs of mice and rats.

Research

JoVE Journal - Engineering
Free Sample

Application of Deep Learning-Based Medical Image Segmentation via Orbital Computed Tomography

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Cited by 4 •

2022

An object segmentation protocol for orbital computed tomography (CT) images is introduced. The methods of labeling the ground truth of orbital structures by using super-resolution, extracting the volume of interest from CT images, and modeling multi-label segmentation using 2D sequential U-Net for orbital CT images are explained for supervised learning.

A Chronic Autoimmune Dry Eye Rat Model with Increase in Effector Memory T Cells in Eyeball Tissue

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Cited by 10 •

2017

This report describes a method to induce chronic experimental autoimmune dry eye in Lewis rats through immunization with an emulsion of rat lacrimal gland extract, ovalbumin, and complete Freund's adjuvant, followed by the injection of lacrimal gland extract and ovalbumin into the forniceal subconjunctiva and lacrimal glands six weeks later.

Research

JoVE Journal - Biology
Free Sample

Volume Segmentation and Analysis of Biological Materials Using SuRVoS (Super-region Volume Segmentation) Workbench

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Cited by 8 •

2017

Segmentation of three-dimensional data from many imaging techniques is a major bottleneck in analysis of complex biological systems. Here, we describe the use of SuRVoS Workbench to semi-automatically segment volumetric data at various length-scales using example datasets from cryo-electron tomography, cryo soft X-ray tomography, and phase contrast X-ray tomography techniques.

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