Focal Stack Reconstruction

Focal stack reconstruction is a computational imaging technique that uses multiple photographs captured at different focus settings to recover scene depth and produce an all-in-focus image. As the focal plane shifts, different regions become sharp while others blur; algorithms compare these focus patterns, estimate a depth map, and fuse the sharpest information across the stack. In engineering, this approach supports three-dimensional measurement, machine vision, microscopy, and inspection of objects with substantial depth variation. It can improve visualization and quantitative analysis when a single image cannot capture both fine detail and extended depth of field.

Focal Stack Reconstruction - Related Videos

Research

JoVE Journal - Biology
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Focal Ca2+ Transient Detection in Smooth Muscle

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2009

Details methods for high-resolution Ca2+ imaging of smooth muscle within isolated organs, including: preparation of the tissue, image acquisition and data analysis.

Research

JoVE Journal - Neuroscience

Functional Interrogation of Adult Hypothalamic Neurogenesis with Focal Radiological Inhibition

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Cited by 8 •

2013

The function of adult-born mammalian neurons remains an active area of investigation. Ionizing radiation inhibits the birth of new neurons. Using computer tomography-guided focal irradiation (CFIR), three-dimensional anatomical targeting of specific neural progenitor populations can now be used to assess the functional role of adult neurogenesis.

Research

JoVE Journal - Biology
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Enhancing Density Maps by Removing the Majority of Particles in Single Particle Cryogenic Electron Microscopy Final Stacks

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Cited by 1 •

2024

An advanced particle selection method for cryo-EM, namely CryoSieve, improves density map resolution by removing a majority of particles in final stacks, as demonstrated through its application on a real-world dataset.

Electroencephalography Recording in a Rat Model of Focal Epilepsy

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2025

This video demonstrates a method for high-resolution EEG recording in a rat model of focal epilepsy. It outlines the steps involved in preparing the rat, connecting a multichannel EEG mini-cap to the rat's scalp, and recording the EEG to identify seizure-related events characteristic of the epilepsy model.

Research

JoVE Journal - Neuroscience

Neuronavigated Focalized Transcranial Direct Current Stimulation Administered During Functional Magnetic Resonance Imaging

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Cited by 13 •

2024

This protocol describes the method of neuronavigated electrode placement for focal, transcranial direct current stimulation (tDCS) administered during functional magnetic resonance imaging (fMRI).

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