Oversampling

Oversampling is the practice of acquiring or processing a signal at a rate substantially higher than the minimum required to represent its highest frequency component. In engineering, an analog-to-digital converter may sample above the Nyquist rate, then use digital filtering and decimation to reduce bandwidth, suppress aliasing, and improve measurement resolution. The additional samples distribute quantization noise across a wider frequency range, allowing filtering to remove more unwanted noise from the signal band. Oversampling supports accurate audio conversion, sensor instrumentation, communications, and imaging systems, although it increases data volume, processing demands, and power consumption.

Oversampling - Related Videos

Research

JoVE Journal - Biology
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Sample Preparation by 3D-Correlative Focused Ion Beam Milling for High-Resolution Cryo-Electron Tomography

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Cited by 17 •

2021

Here, we present a pipeline for 3D-correlative focused ion beam milling on guiding the preparation of cellular samples for cryo-electron tomography. The 3D position of fluorescently tagged proteins of interest is first determined by cryo-fluorescence microscopy, and then targeted for milling. The protocol is suitable for mammalian, yeast, and bacterial cells.

Research

JoVE Journal - Neuroscience
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Molecular Imaging of Human Brain Organoids Using Mass Spectrometry

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Cited by 2 •

2024

An advanced method was developed for mass spectrometry imaging (MSI) of brain organoids that allows mapping metabolite distributions within these models. This technology offers insights into brain metabolic pathways and metabolite signatures during early development and in disease, promising a deeper understanding of the human brain function.

Characterization of Neuromuscular Junctions in Mice by Combined Confocal and Super-Resolution Microscopy

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Cited by 2 •

2021

This protocol describes a method for morphometric analysis of neuromuscular junctions by combined confocal and STED microscopy that is used to quantify pathological changes in mouse models of SMA and ColQ-related CMS.

Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy

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Cited by 12 •

2014

Here we illustrate the protocol for imaging by two-color STED nanoscopy the cytotoxic immune synapse of NK cells recapitulated on glass. Using this method we obtain sub-100 nm resolution of synapse proteins and the cytoskeleton.

Quantitative Locomotion Study of Freely Swimming Micro-organisms Using Laser Diffraction

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Cited by 10 •

2012

Microscopic organisms like the free-swimming nematode C. elegans, live and behave in a complex three-dimensional environment. We report on a novel approach that provides analysis of C. elegans using diffraction patterns. This approach consists of tracking the temporal periodicity of diffraction patterns generated by directing laser light through a cuvette.

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