Stirrups

In structural engineering, stirrups are closed or open-loop reinforcing bars placed transversely around the main longitudinal reinforcement in concrete members. They help resist shear by crossing potential diagonal cracks, while also holding the primary bars in position and confining the concrete core, especially in columns and beam-column regions. Properly spaced and anchored stirrups improve load transfer, crack control, ductility, and resistance to brittle shear failure. Engineers select their diameter, shape, spacing, and detailing according to expected loads, member geometry, concrete properties, and applicable design codes, making stirrups essential to the safety and durability of reinforced-concrete structures.

Stirrups - Related Videos

Research

JoVE Journal - Biochemistry
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Automated Protocols for Macromolecular Crystallization at the MRC Laboratory of Molecular Biology

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Cited by 14 •

2018

Automated systems and protocols for the routine preparation of a large number of screens and nanoliter crystallization droplets for vapor diffusion experiments are described and discussed.

Developing Custom Chinese Hamster Ovary-host Cell Protein Assays using Acoustic Membrane Microparticle Technology

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Cited by 5 •

2011

Development of custom assays on the ViBE platform for more sensitive, reproducible, automated results in complex matrices is described. The universal cartridge allows assays to be easily adapted for use with custom assays. This versatility enables rapid development and validation of novel assays or automated versions of existing manual assays, exemplified in this video.

Research

JoVE Journal - Biochemistry

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

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Cited by 2 •

2023

This protocol provides a rapid and size-specific isolation method for small extracellular vesicles by optimizing the size of the air spray nozzle, sheath fluid pressure, sample flow pressure, voltage, gain, and triggering threshold parameters.

Research

JoVE Journal - Biology
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The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis

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Cited by 25 •

2010

We apply label-free protein interaction analysis using Biacore X100 for structure-function analysis of the binding of several cystatin B mutants to papain through kinetic characterization. Calibration-free concentration analysis (CFCA) measures the concentration of protein with retained binding activity without the need for a standard curve. We show that confirmation of concentrations using CFCA increases the reliability of the kinetic analysis and that kinetic constants can reliably be...

Headspace Gas Chromatography: A Technique to Assess Ethanol Concentration in Zebrafish Embryos

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2025

This video demonstrates the use of headspace gas chromatography to determine the ethanol concentrations in zebrafish embryos. This method provides a useful tool for qualitative and quantitative analysis of volatile organic compounds.

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