Fixation Hole Training

Fixation hole training is a behavioral procedure that teaches an animal to direct and maintain its gaze on a defined location, creating controlled conditions for studying visual behavior. Using operant conditioning, the subject receives reinforcement when it places its eyes within the required fixation area and holds gaze for a specified interval, while departures can end or reset a trial. This training supports precise measurement of eye movements, visual attention, and stimulus responses in behavioral neuroscience. It also improves the reliability of experiments involving eye tracking, neural recording, and controlled presentation of visual stimuli.

Fixation Hole Training - Related Videos

Research

JoVE Journal - Medicine

Fixation and Operational Method for Abdominal Massage in T2DM Mice

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2025

This study introduces an abdominal massage device for mice that replicates manual massage while minimizing stress and tissue damage. The device significantly lowers blood glucose levels, improves lipid metabolism, and enhances insulin sensitivity in type 2 diabetes mice, offering a promising, non-invasive therapeutic approach with clinical potential.

Research

JoVE Journal - Neuroscience
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Whole Animal Perfusion Fixation for Rodents

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Cited by 531 •

2012

Here we describe a low-cost, rapid, controlled and uniform fixation procedure using 4% paraformaldehyde perfused via the vascular system: through the heart of the rat to obtain the best possible preservation of the brain.

Education

JoVE Core - Physics

Detection of Black Holes

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2023

Although black holes were theoretically postulated in the 1920s, they remained outside the domain of observational astronomy until the 1970s. Their closest cousins are neutron stars, which are composed almost entirely of neutrons packed against each other, making them extremely dense. A neutron star has the same mass as the Sun but its diameter is only a few kilometers. Therefore, the escape velocity from their surface is close to the speed of light. Not until the 1960s, when the first neutron...

Acrylic Resin Molding Based Head Fixation Technique in Rodents

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Cited by 4 •

2016

Traditional head fixation techniques have used metallic frames attached on the skull as an interface for head fixation apparatus. This protocol demonstrates a frameless, acrylic resin molding based head fixation technique for behavioral tasks in rodents.

Research

JoVE Journal - Biology

Stabilizing a Femur Osteotomy with a Plate Fixation in Ambystoma mexicanum

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2024

A protocol for femoral osteotomy surgery with the use of internal plate fixation in mature axolotls is presented. The procedure can be used to perform comparative studies on limb regeneration and fracture healing in aquatic amphibians.

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