Fluorescent Pegylation
Fluorescent Pegylation - Related Videos
10:18
Making Conjugation-induced Fluorescent PEGylated Virus-like Particles by Dibromomaleimide-disulfide Chemistry
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Cited by 10 •
2018
Here, we present a procedure to fluorescently functionalize the disulfides on Qβ VLP with dibromomaleimide. We describe Qβ expression and purification, the synthesis of dibromomaleimide-functionalized molecules, and the conjugation reaction between dibromomaleimide and Qβ. The resulting yellow fluorescent conjugated particle can be used as a fluorescence probe inside cells.
08:51
Visualization of Surface-tethered Large DNA Molecules with a Fluorescent Protein DNA Binding Peptide
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Cited by 7 •
2016
We present an approach for visualizing fluorescent protein DNA binding peptide (FP-DBP)-stained large DNA molecules tethered on the polyethylene glycol (PEG) and avidin-coated glass surface and stretched with microfluidic shear flows.
08:28
Acyl-PEGyl Exchange Gel Shift Assay for Quantitative Determination of Palmitoylation of Brain Membrane Proteins
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Cited by 7 •
2020
Palmitoylation entails the incorporation of a 16-carbon palmitate moiety to cysteine residues of target proteins in a reversible manner. Here, we describe a biochemical approach, the acyl-PEGyl exchange gel shift (APEGS) assay, to investigate the palmitoylation state of any protein of interest in mouse brain lysates.
09:24
Synthesis, Hemoglobin Encapsulation and Biorthogonal PEGylation in Hierarchically Porous UiO-66 Nanoparticles for Oxygen Delivery Applications
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2026
This protocol details the synthesis, hemoglobin encapsulation, and surface PEGylation of hierarchically porous UiO-66 nanoparticles designed for oxygen delivery. The procedure combines defect engineering with strain-promoted biorthogonal conjugation to generate highly permeable and physiologically stable carriers suitable for biomedical applications.
10:59
Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy
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Cited by 1 •
2024
This article describes the dual-color labeling of long RNAs at termini positions and their surface immobilization via encapsulation in phospholipid vesicles for single-molecule FRET TIRF microscopy applications. Combining these techniques enables precise visualization and analysis of RNA dynamics at the single-molecule level.
FAQs
Reactive groups placed on the PEG chain determine how it attaches to a target. They form stable covalent bonds with complementary chemical groups on proteins, nanoparticles, cells, surfaces, or engineered materials. This compatibility is central to conjugation efficiency because the PEG-fluorophore must connect securely enough for subsequent visualization, quantification, or evaluation of the modified interface.
The PEG portion contributes functional effects that can be evaluated, including changes in stability, solubility, and biological recognition. The fluorescent label supplies an optical signal that helps track where the modified material or molecule is distributed and estimate how much attachment occurred. Together, these components connect structural modification with measurable behavior in bioengineered systems.
Fluorescence can indicate that labeled PEG is associated with a target, but the key interpretation is whether attachment occurred efficiently and through stable bonding. Measuring conjugation efficiency helps distinguish successful modification from limited attachment and supports comparisons among proteins, nanoparticles, cells, surfaces, or biomaterials. This information is useful when assessing whether a designed interface performs as intended.
Attaching PEG may alter how a molecule, particle, or material is recognized biologically, while the fluorophore makes the modified component easier to follow. Comparing its distribution or interactions with the unmodified design can therefore connect PEG-associated changes to biological recognition. In bioengineering, this supports evaluation of whether an engineered interface maintains the desired behavior after modification.
A general workflow begins by selecting a PEG chain that carries both a fluorescent label and a reactive group compatible with the target. The labeled PEG is then brought into contact with a protein, nanoparticle, cell, surface, or biomaterial so covalent attachment can occur. Fluorescence measurements can subsequently assess conjugation efficiency and distribution.
Researchers can apply it when they need to examine how PEG modification affects an engineered biological interface while also tracking the modified component. Relevant uses include drug-delivery systems, diagnostic platforms, biosensors, and other biomaterials. Fluorescent readouts help evaluate molecular distribution and conjugation, while functional assessment addresses changes in stability, solubility, or biological recognition.
The approach can provide evidence about whether fluorescent PEG attached to the intended molecule or material, how the modified component is distributed, and how PEG-associated properties change. These outcomes help guide design decisions for drug-delivery systems and diagnostic platforms. In biosensors and related interfaces, the same information supports evaluation of material interactions and engineered biological performance.