Irreversible Electroporation
Irreversible Electroporation - Related Videos
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Irreversible Electroporation: A Technique for Ablating Pancreatic Tumor in Mouse
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2023
This video describes the procedure for performing irreversible electroporation (IRE) for non-thermal ablation of pancreatic tumors in mice. Both subcutaneous (SQ) and orthotopic tumors can be successfully treated with IRE in a highly controlled setting, facilitating various studies post-procedure.
08:05
A Syngeneic Pancreatic Cancer Mouse Model to Study the Effects of Irreversible Electroporation
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Cited by 10 •
2018
Irreversible electroporation (IRE) is a non-thermal ablation technique used for the treatment of locally advanced pancreatic cancer. Being a relatively new technique, the effects of IRE on the tumor growth are poorly understood. We have developed a syngeneic mouse model that facilitates studying the effects of IRE on pancreatic cancer.
06:17
Covalent Fragment Screening Using the Quantitative Irreversible Tethering Assay
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2025
The quantitative irreversible tethering (qIT) assay is a fluorescence-based method of identifying covalent fragments that selectively engage with a target protein. Here, a detailed protocol is outlined to describe the qIT assay, with example results included.
14:05
A Murine Model of Irreversible and Reversible Unilateral Ureteric Obstruction
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Cited by 47 •
2014
The murine model of irreversible unilateral ureteric obstruction (UUO) is presented together with the model of reversible UUO in which the ureteric obstruction is relieved by anastomosis of the severed ureter into the bladder. These models enable the study of renal inflammation and scarring as well as tissue remodeling.
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Reversible and Irreversible Processes
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2023
The thermodynamic processes can be classified into reversible and irreversible processes. The processes that can be restored to their initial state are called reversible processes. It is only possible if the process is in quasi-static equilibrium, i.e., it takes place in infinitesimally small steps, and the system remains at equilibrium However, these are ideal processes and do not occur naturally. An ideal system undergoing a reversible process is always in thermodynamic equilibrium within...
FAQs
The critical electric-field threshold determines whether membrane damage becomes permanent. Above it, nanoscale pores form in the lipid bilayer, and continued pore expansion can overwhelm membrane-repair capacity. The resulting ion imbalance disrupts cellular homeostasis and leads to cell death. This threshold-based response helps explain why changing pulse conditions can alter which cells or regions are affected.
Pulse amplitude, duration, and number jointly shape the electric exposure delivered to tissue. Tissue properties also influence how that exposure is distributed, so the same pulse settings do not necessarily produce the same treated region in every sample. Controlling these variables is therefore central to achieving localized effects and interpreting differences in cellular response.
Irreversible Electroporation differs from approaches that depend primarily on thermal damage because its central injury mechanism is permanent membrane disruption. This nonthermal basis can support cell removal while preserving surrounding structural components. In bioengineering, that distinction matters when researchers need to alter a defined region without making heat the main driver of the intervention.
Cell death follows a linked sequence rather than a single pore-forming event. Once membrane pores fail to close, ions move out of balance, cellular homeostasis is lost, and the cell can no longer maintain its normal internal conditions. Connecting pore behavior to homeostatic failure provides a mechanistic way to interpret the outcome of an applied electric field.
A practical experimental design begins by matching pulse amplitude, duration, and number to the desired treated region, then accounting for tissue properties that affect field distribution. Researchers can evaluate the resulting cellular response and adjust exposure conditions accordingly. This parameter-based approach links electrical input to spatial selectivity rather than treating pulse delivery as a fixed recipe.
In cancer research, the method can be investigated as a way to remove selected tissue regions through nonthermal cellular injury. Its value is not limited to the cell-death endpoint: the ability to localize treatment and preserve surrounding structural components makes it relevant to studies of targeted biomedical interventions. The exact outcome depends on exposure settings and tissue properties.
For engineered tissue processing, Irreversible Electroporation offers a way to produce localized cellular removal while retaining surrounding structural components. That combination is relevant when the biological architecture around a targeted region must remain available for further study or intervention. More broadly, the technique connects electrical-field control with spatially selective manipulation of living tissue in bioengineering.