Electron Microscopy
Electron Microscopy - Related Videos
11:16
Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
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Cited by 12 •
2016
A method is described whereby quantum dot (QD) nanoparticles can be used for correlative immunocytochemical studies of epoxy embedded human pathology tissue. We employ commercial antibody fragment conjugated QDs that are visualized by widefield fluorescence light microscopy and transmission electron microscopy.
08:57
Focussed Ion Beam Milling and Scanning Electron Microscopy of Brain Tissue
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Cited by 101 •
2011
This protocol describes how resin embedded brain tissue can be prepared and imaged in the three dimensions in the focussed ion beam, scanning electron microscope.
09:53
Micropatterning Transmission Electron Microscopy Grids to Direct Cell Positioning within Whole-Cell Cryo-Electron Tomography Workflows
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Cited by 29 •
2021
The goal of this protocol is to direct cell adhesion and growth to targeted areas of grids for cryo-electron microscopy. This is achieved by applying an anti-fouling layer that is ablated in user-specified patterns followed by deposition of extra-cellular matrix proteins in the patterned areas prior to cell seeding.
09:20
Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy
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Cited by 16 •
2011
This study describes a method that allows the rapid and clear diagnosis of plant virus diseases in about half a day by using a combination of microwave assisted plant sample preparation for transmission electron microscopy and negative staining methods.
07:52
Cryo-Electron Microscopy Screening Automation Across Multiple Grids Using Smart Leginon
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Cited by 3 •
2023
Cryo-electron microscopy (cryoEM) multi-grid screening is often a tedious process that demands hours of attention. This protocol shows how to set up a standard Leginon collection and Smart Leginon Autoscreen to automate this process. This protocol can be applied to the majority of cryoEM holey foil grids.
FAQs
The principal distinction is whether the analysis emphasizes internal organization or surface architecture. Transmission electron microscopy uses ultrathin sections so electrons can pass through a specimen, supporting detailed views of internal structures such as organelles. Scanning electron microscopy instead detects electrons emitted from the surface, producing three-dimensional views that emphasize topography. Researchers choose between them according to the biological feature of interest.
Preparation is matched to the way each method forms an image. Transmission electron microscopy requires fixation, dehydration, embedding, and sectioning to create ultrathin specimens suitable for electron passage. Scanning electron microscopy may require fixation, dehydration, and coating so the specimen surface can be examined. These differing workflows determine whether the resulting image emphasizes internal structure or surface form.
Its nanometer-scale resolution allows investigators to examine structural relationships that conventional light microscopy cannot resolve. This scale is especially useful for analyzing cell ultrastructure, organelles, tissues, and pathogens, where organization depends on details smaller than those visible with ordinary microscopy. The resulting images connect cellular or material architecture with the specific biological structures being investigated.
A typical transmission workflow includes fixation, dehydration, embedding, and sectioning before imaging. The specimen is first stabilized, water is removed, and the material is embedded so ultrathin sections can be produced. Those sections allow electrons to pass through the sample and generate detailed internal images. This procedure is therefore suited to examining cellular organization and organelles.
Scanning electron microscopy preparation focuses on making the specimen surface suitable for imaging. The workflow can include fixation, dehydration, and coating, followed by detection of electrons emitted from the surface. Because the method records surface information, the resulting image emphasizes three-dimensional topography rather than internal sections. This makes it appropriate when surface architecture is the main research question.
Electron microscopy supports investigations of cell ultrastructure, organelles, tissues, pathogens, and biomaterials. Transmission images can reveal how internal components are arranged, whereas scanning images can show the form and surface organization of a specimen. By selecting the imaging mode according to the structure of interest, researchers can examine biological organization at scales unavailable to conventional microscopy.