Automated Isolation
Automated Isolation - Related Videos
08:06
Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays
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Cited by 1 •
2016
Peptide adsorption to plasticware during traditional tip-based serial dilutions can significantly impact potency determination and confound the understanding of structure-activity relationships used for lead identification and lead optimization phases of drug discovery. Here methods for automated acoustic non-contact serial dilution of peptide samples are described.
07:12
A Simple, Quick, and Partially Automated Protocol for the Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nucleus Sequencing
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Cited by 2 •
2023
The study describes a simple, quick, and partially automated protocol to isolate high-quality nuclei from frozen mammalian tissues for downstream single nuclei RNA sequencing.
13:49
Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates
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Cited by 6 •
2017
Biopanning bacterial display libraries is a proven technique for discovery of peptide affinity reagents, a robust alternative to antibodies. The semi-automated sorting method herein has streamlined biopanning to decrease the occurrence of false positives. Here we illustrate the thought process and techniques applied in evaluating candidates and minimizing downstream analysis.
09:12
Isolation of Adeno-Associated Viral Vectors Through a Single-Step and Semi-Automated Heparin Affinity Chromatography Protocol
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Cited by 8 •
2024
This manuscript describes a detailed protocol for the generation and purification of adeno-associated viral vectors using an optimized heparin-based affinity chromatography method. It presents a simple, scalable, and cost-effective approach, eliminating the need for ultracentrifugation. The resulting vectors exhibit high purity and biological activity, proving their value in preclinical studies.
06:08
Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
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Cited by 2 •
2023
This protocol describes the semi-automated isolation of the stromal vascular fraction (SVF) from murine adipose tissue to obtain preadipocytes and achieve adipocyte differentiation in vitro. Using a tissue dissociator for collagenase digestion reduces experimental variation and increases reproducibility.
FAQs
These capture materials provide the separation surface within an automated workflow. After sample lysis, genetic material is retained on magnetic particles or a specialized membrane, while washing removes unwanted contaminants. Elution then transfers the purified DNA or RNA into a clean solution. The capture and release stages make the preparation suitable for later genetic analysis.
Each stage contributes a different requirement for usable genetic material. Lysis prepares the biological sample, capture retains nucleic acids, washing removes contaminants, and elution releases the material into a clean solution. Automated control of volumes and timing helps coordinate these conditions consistently, which supports reliable preparation for downstream genotyping, sequencing, or gene expression studies.
Robotic systems apply programmed volumes, timing, and handling across samples rather than relying entirely on manual execution. This standardization reduces differences introduced during processing and limits unnecessary sample handling. Consistent operation is especially valuable when many samples must be prepared for comparison, because processing variability and contamination risks can otherwise affect downstream genetic results.
A typical workflow begins with sample lysis, followed by nucleic-acid capture on magnetic particles or a specialized membrane. The system then performs washing steps to remove contaminants and elutes the retained material into a clean solution. Throughout the procedure, the instrument manages volumes and timing while maintaining sample tracking, creating prepared DNA or RNA for subsequent analysis.
The workflow requires an instrument capable of coordinating robotic sample processing, along with a capture medium such as magnetic particles or a specialized membrane. Samples also pass through lysis, washing, and elution stages, with the final material collected in a clean solution. Sample tracking is an additional operational component that helps maintain identity and workflow consistency.
Automated isolation is useful when genetics workflows require consistent preparation across multiple biological samples. The resulting DNA or RNA can support genotyping, sequencing, and gene expression studies, as well as diagnostic research. Its ability to increase throughput while reducing manual handling makes it relevant when sample numbers, processing consistency, and contamination control are important.