Active Galactic Nuclei
Active Galactic Nuclei - Related Videos
08:16
Fluorescence-Activated Nuclei Negative Sorting of Neurons Combined with Single Nuclei RNA Sequencing to Study the Hippocampal Neurogenic Niche
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2022
Presented here is a method to sequence single nuclei isolated from the mouse dentate gyrus that excludes most neurons through fluorescence-activated nuclei (FAN)-sorting. This approach generates high-quality expression profiles and facilitates the study of most other cell types represented in the niche, including scarce populations such as neural stem cells.
08:48
Complementation of Splicing Activity by a Galectin-3 - U1 snRNP Complex on Beads
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2020
This article describes the experimental procedures for (a) depletion of U1 snRNP from nuclear extracts, with concomitant loss of splicing activity; and (b) reconstitution of splicing activity in the U1-depleted extract by galectin-3 - U1 snRNP particles bound to beads covalently coupled with anti-galectin-3 antibodies.
03:14
Isolating Cell Populations from Brain Tissue Using Fluorescence-Activated Nuclei Sorting
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2025
This video demonstrates the procedure for isolating and sorting nuclei from fresh-frozen human brain tissue using fluorescence-activated nuclei sorting (FANS), which enables the study of different cell populations for various neurological applications.
03:05
Gradient Centrifugation Based Purification of Nuclei: A Technique to Obtain Ultrapure Fraction of Intact Nuclei Using Density Gradient Centrifugation
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2023
In this video, we describe a simple and efficient protocol using gradient centrifugation to isolate single nuclei. Once isolated, these nuclei can be used for sequencing and genome analysis.
05:59
Isolation of Nuclei from Human Intermuscular Adipose Tissue and Downstream Single-Nuclei RNA Sequencing
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Cited by 2 •
2024
The biology of intermuscular adipose tissue (IMAT) is largely unexplored due to the limited accessibility of human tissue. Here, we present a detailed protocol for nuclei isolation and library preparation of frozen human IMAT for single nuclei RNA sequencing to identify the cellular composition of this unique adipose depot.
FAQs
As gas and dust move toward a central supermassive black hole, gravitational energy is converted into intense radiation through the rapidly rotating accretion disk. The disk therefore connects the inward motion of matter with observable emission. Studying this energy conversion helps physicists investigate how black hole growth produces extreme luminosity in galactic centers.
Magnetic fields can help launch collimated jets and powerful outflows from the region surrounding the black hole. Collimated means that the material and energy are directed into relatively narrow structures rather than spreading uniformly. These features provide evidence that magnetic processes participate in shaping how energy and matter leave the central environment.
AGN emit from radio waves through X-rays, giving physicists access to radiation across a broad electromagnetic range. This wide emission makes them valuable laboratories for examining black hole growth, plasma physics, and relativistic processes. Considering the full range of emission helps connect the central accretion environment with the different physical phenomena it produces.
Researchers treat the radio-to-X-ray emission of AGN as evidence of several linked physical processes operating near a supermassive black hole. Radiation from the accretion environment, together with signatures associated with jets and outflows, allows AGN to serve as laboratories for plasma physics and relativistic processes. This makes them useful for studying extreme conditions in astrophysics.
The intense radiation produced as material falls toward a supermassive black hole provides a way to study black hole growth. Because the accretion disk converts gravitational energy into radiation, its activity links the supply of gas and dust to a measurable luminous output. AGN therefore connect the physics of infalling matter with the development of central black holes.
Radiation and powerful outflows from an active nucleus can influence star formation and the evolution of its host galaxy. This creates a physical connection between processes occurring near a compact central black hole and changes occurring across much larger galactic scales. AGN are therefore important for investigating how small-scale accretion can affect large-scale cosmic structure.