Dna Detection
Dna Detection - Related Videos
06:19
Comet Assay for DNA Damage Detection in Cells
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2025
The video demonstrates the comet assay for detecting DNA damage following exposure to harmful agents. Under alkaline conditions, the DNA unwinds and denatures, and upon electrophoresis, the undamaged and damaged DNA migrate differently in the agarose gel, forming a comet-shaped structure. The slowly migrating undamaged DNA forms the circular comet head, while the rapidly migrating damaged DNA fragments form the elongated comet tail.
09:32
ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza
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Cited by 4 •
2011
ampliPHOX colorimetric detection technology is presented as an inexpensive alternative to fluorescence detection for microarrays. Based on photopolymerization, ampliPHOX produces solid polymer spots visible to the naked eye in just a few minutes. Results are then imaged and automatically interpreted with a simple yet powerful software package.
05:12
Extraction of Total DNA from Leaves for Detection of Bacterial Infection
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2026
Source: Chen, H., et al. Specific and Accurate Detection of the Citrus Greening Pathogen Candidatus liberibacter spp. Using Conventional PCR on Citrus Leaf Tissue Samples. J. Vis. Exp. (2018)This video demonstrates the extraction of total genomic DNA from citrus leaf tissue infected with a pathogenic bacterium. The protocol enables reliable DNA recovery for downstream PCR-based detection of the phloem-limited pathogen.
07:46
Detection of DNA Double-Stranded Breaks in Mouse Oocytes
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Cited by 2 •
2023
Maintaining oocyte genome integrity is necessary to ensure the genetic fidelity in the resulting embryo. Here, we present an accurate protocol for detecting DNA double-strand breaks in mammalian female germ cells.
10:12
Quantitative Detection of DNA-Protein Crosslinks and Their Post-Translational Modifications
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Cited by 3 •
2023
The present protocol highlights a modified method to detect and quantify DNA-protein crosslinks (DPCs) and their post-translational modifications (PTMs), including ubiquitylation, SUMOylation, and ADP-ribosylation induced by topoisomerase inhibitors and by formaldehyde, thereby allowing the study of the formation and repair of DPCs and their PTMs.
FAQs
Complementary base pairing allows a probe sequence to recognize a matching target sequence rather than unrelated DNA. This molecular recognition provides the selectivity needed to distinguish targets within complex biological or environmental samples. Probe design and the binding conditions therefore influence whether the system produces a specific signal or responds to non-target sequences.
Amplification methods increase the amount of target-derived material available for measurement, while fluorescent, colorimetric, or electrical labels translate molecular binding into an observable output. These strategies address different stages of sensing: amplification can improve detectability, and labeling determines how the binding event is read. Their combination supports sensitive measurements across engineered systems.
Performance depends on how effectively the probe recognizes its target, how the binding event is converted into a signal, and how the system handles the sample. Engineering improvements commonly focus on sensitivity, selectivity, speed, and portability together, because increasing one characteristic alone may not produce a practical device for clinical, industrial, or field-based use.
A typical workflow brings a sample into contact with a matching probe, allows target recognition through complementary binding, and converts that interaction into a measurable output. The readout may be fluorescent, colorimetric, or electrical. In engineered platforms, microfluidics can help organize sample handling and sensing steps, while amplification or labels can strengthen the resulting signal.
Applications include pathogen screening, environmental monitoring, genetic analysis, and diagnostic systems. Portable formats are especially relevant when measurements must be made outside centralized laboratories. By combining molecular recognition with compact fluidic, material, or electronic components, engineered devices can support faster and more accessible analysis in clinical, industrial, and field-based settings.
Engineering integrates the recognition chemistry with microfluidics, nanomaterials, and electronic readouts to create more compact and functional sensing platforms. These components can support portability, signal measurement, and sample handling within a unified system. Such integration is important for translating DNA detection from a laboratory procedure into deployable tools for monitoring and screening.