Sample Holder Assembly
Sample Holder Assembly - Related Videos
07:55
An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering
0 Views •
Cited by 4 •
2019
A novel sample holder for macromolecular X-ray crystallography along with a suitable handling protocol is presented. The system allows crystal growth, crystal soaking and in situ diffraction data collection at both, ambient and cryogenic temperature without the need of any crystal manipulation or mounting.
11:36
Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell
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Cited by 21 •
2009
The analytical ultracentrifuge (AUC) sample cell holds sample and reference buffer and during experiments and is exposed to high vacuum and rotor speeds up to 60,000 rpm. This video will demonstrate the rigorous attention to detail necessary for assembly, loading and alignment of this very important component of an AUC experiment.
05:41
Live-Cell Imaging of Intact Ex Vivo Globes Using a Novel 3D Printed Holder
0 Views •
Cited by 2 •
2022
The present work describes a novel experimental protocol that utilizes a 3D printed holder to enable high-resolution live cell imaging of enucleated globes. Through this protocol, the cellular calcium signaling activity in wounded corneal epithelium from ex vivo globes can be observed in real time.
08:28
In situ TEM of Biological Assemblies in Liquid
0 Views •
Cited by 8 •
2013
Here we describe a procedure to image viral complexes in liquid at nanometer resolution using a transmission electron microscope.
Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice
0 Views •
Cited by 6 •
2022
Here protocols are described to prepare virus assemblies suitable for liquid-EM and cryo-EM analysis at the nanoscale using transmission electron microscopy.
FAQs
Stability comes from controlling three relationships: the sample’s orientation, its contact with the holder, and any applied load. Supports and alignment guides establish position, while clamps can resist movement. Seals may help maintain a controlled interface. Balancing these features is important because excessive or uneven restraint can introduce unintended stresses into the test.
Sample Holder Assembly affects whether measurements represent the specimen or unwanted motion and loading effects. Movement can change the sample’s position during testing, while unintended stresses can alter its response. Contamination may also compromise the interface. A stable arrangement reduces these sources of variation, supporting repeatable experiments and more meaningful comparisons between samples or tests.
Each feature addresses a different part of specimen control. Supports provide a physical base, clamps help secure the sample, and alignment guides help establish its intended orientation. Seals contribute to controlled contact and environmental management. Combining these elements allows the holder to accommodate the required position, restraint, accessibility, and instrument interface without relying on one component for every function.
Begin by considering the sample geometry, the instrument interface, and the required orientation. Arrange supports or guides to establish position, then use clamps or other securing features to limit movement without creating unintended stresses. Add seals when environmental control or a defined interface is needed. Finally, confirm that the assembled arrangement permits the intended measurement, test, or processing access.
Engineers use Sample Holder Assembly when materials, devices, or manufactured parts must be characterized under controlled positioning and contact conditions. It can support measurement, testing, or processing across different sample geometries and instrument configurations. The resulting arrangement helps researchers compare specimens consistently while reducing errors caused by movement, contamination, misalignment, or uncontrolled loading.
Holder design must balance secure positioning with access to the regions needed for measurement, testing, or processing. Features that improve restraint should not unnecessarily block the instrument interface or specimen surface. Seals and related interfaces can support environmental control, while the overall arrangement must remain compatible with the sample geometry and instrument so the intended procedure can be performed reliably.