Mitochondrial Oxygenation
Mitochondrial Oxygenation - Related Videos
05:59
Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals
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Cited by 3 •
2023
Here, we present a compilation of assays to directly measure mitochondrial function in mammalian cells independently of their ability to consume molecular oxygen.
05:47
Oxygen Flux Measurements to Evaluate Mitochondrial Respiration in Living and Permeabilized Cells
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2026
Source: Elettra Leo1, Luiza H.D. Cardoso1, Sabine Schmitt1, Erich Gnaiger11Oroboros InstrumentsThis video explains how oxygen flux is used to assess mitochondrial respiration in living and permeabilized mammalian cells. It demonstrates the sequential use of digitonin, metabolic substrates, ADP, an uncoupler, and an inhibitor to characterize different respiratory states. The resulting oxygen flux profile reveals mitochondrial function and energy-coupling efficiency under defined conditions.
09:56
Determination of Mitochondrial Membrane Potential and Reactive Oxygen Species in Live Rat Cortical Neurons
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Cited by 230 •
2011
We demonstrate application of the fluorescence indicator, TMRM, in cortical neurons to determine the relative changes in TMRM fluorescence intensity before and after application of a specific stimulus. We also show application of the fluorescence probe H2DCF-DA to assess the relative level of reactive oxygen species in cortical neurons.
08:54
Development of a Mobile Mitochondrial Physiology Laboratory for Measuring Mitochondrial Energetics in the Field
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Cited by 2 •
2021
We designed and constructed a mobile laboratory to measure respiration rates in isolated mitochondria of wild animals captured at field locations. Here, we describe the design and outfitting of a mobile mitochondrial laboratory and the associated laboratory protocols.
07:24
Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
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Cited by 5 •
2023
Pyrosequencing assays enable the robust and rapid genotyping of mitochondrial DNA single nucleotide polymorphisms in heteroplasmic cells or tissues.
FAQs
When oxygen availability falls, Complex IV has less terminal electron acceptor capacity, which can restrict electron transport through the respiratory chain. That limitation reduces proton-gradient formation across the inner mitochondrial membrane and consequently weakens the driving force for ATP synthesis. In engineered tissues, this mechanism helps explain why poorly oxygenated regions may show impaired metabolism, reduced performance, or lower cell survival.
Oxygen conditions experienced by mitochondria depend on delivery through the surrounding system as well as cellular use. A bioengineered construct may therefore require analysis of oxygen transport in the tissue and oxygen utilization within its cells. Considering both scales helps connect local oxygen availability with mitochondrial function, overall metabolic behavior, and the performance of the engineered tissue.
Hypoxia models create conditions in which oxygen availability is reduced, allowing researchers to examine how mitochondrial function responds to oxygen limitation. These models can connect altered oxygen conditions with changes in cell survival, metabolic function, and tissue performance. In bioengineering, they also provide a controlled way to test whether a tissue design or material better supports function in poorly oxygenated environments.
Mitochondrial oxygenation is influenced by how oxygen is delivered through the system, how cells use it, and whether the construct develops poorly oxygenated regions. Perfusion systems can help control delivery, while engineered-tissue models can reveal how tissue organization affects oxygen conditions. Measuring these variables together helps identify links between oxygen availability, mitochondrial activity, and tissue performance.
A typical assessment combines an engineered-tissue or cellular model with oxygen sensors and, when needed, a perfusion system that controls oxygen delivery. Researchers can then examine oxygen conditions alongside indicators of metabolic function, cell survival, or tissue performance. This combined approach helps determine whether a design maintains adequate oxygenation rather than evaluating oxygen availability in isolation.
Perfusion systems are useful when researchers need to control or examine oxygen delivery within an engineered tissue model. By incorporating perfusion into the experimental design, they can study how oxygen transport relates to mitochondrial use and tissue behavior. This is especially relevant for investigating poorly oxygenated environments and for evaluating engineered approaches intended to support cellular function under those conditions.
Oxygen-sensor measurements provide information about oxygen conditions within the experimental system, helping researchers identify whether regions may be adequately supplied or poorly oxygenated. When paired with measurements of cell survival, metabolic function, or tissue performance, the data can show how oxygen availability affects biological behavior. The resulting information supports refinement of tissue designs, models, and biomaterials.
Understanding mitochondrial oxygenation helps bioengineers evaluate whether a therapy, biomaterial, or engineered tissue can support cells in environments where oxygen is limited. Oxygen sensors, perfusion systems, and hypoxia models allow designs to be tested against cellular and tissue outcomes. This connects oxygen transport with practical measures of function and can guide strategies for improving performance in poorly oxygenated settings.