Cell-material Interaction
Cell-material Interaction - Related Videos
06:50
Sandwich-like Microenvironments to Harness Cell/Material Interactions
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Cited by 3 •
2015
The following protocol describes the procedure to assemble sandwich-like cultures to be used as an intermediate stage between bi-dimensional (2D) and three-dimensional (3D) cellular environments. The engineered systems can have applications in microscopy, biomechanics, biochemistry and cell biology assays.
13:02
Microfabrication of Nanoporous Gold Patterns for Cell-material Interaction Studies
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Cited by 16 •
2013
We report on techniques to micropattern nanoporous gold thin films via stencil printing and photolithography, as well as methods to culture cells on the microfabricated patterns. In addition, we describe image analysis methods to characterize morphology of the material and the cultured cells using scanning electron and fluorescence microscopy techniques.
12:22
Biomimetic Materials to Characterize Bacteria-host Interactions
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Cited by 8 •
2015
Bacterial attachment to host cells is a key step during host colonization and infection. This protocol describes the generation of polymer-coupled recombinant adhesins as biomimetic materials which allow analysis of the contribution of individual adhesins to these processes, independent of other bacterial factors.
07:37
Revealing Dynamic Processes of Materials in Liquids Using Liquid Cell Transmission Electron Microscopy
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Cited by 9 •
2012
We have developed a self-contained liquid cell, which allows imaging through liquids using a transmission electron microscope. Dynamic processes of nanoparticles in liquids can be revealed in real time with sub-nanometer resolution.
11:23
Material Constants
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2023
Source: Roberto Leon, Department of Civil and Environmental Engineering, Virginia Tech, Blacksburg, VA In contrast to the production of cars or toasters, where millions of identical copies are made and extensive prototype testing is possible, each civil engineering structure is unique and very expensive to reproduce (Fig.1). Therefore, civil engineers must extensively rely on analytical modeling to design their structures. These models are simplified abstractions of reality and are used to...
FAQs
Proteins and ions from surrounding fluids can adsorb onto a material surface and create the immediate chemical interface encountered by cells. Cell-membrane receptors bind molecules present at this interface rather than responding only to the underlying material. The adsorbed layer therefore helps regulate attachment, signaling, and later cellular responses.
Surface chemistry, electrical charge, wettability, roughness, and stiffness can change how cells attach and spread. These factors affect the interfacial environment in which receptors engage surface-associated molecules and cells reorganize their cytoskeleton. As a result, modifying a surface property can alter adhesion, proliferation, or differentiation even when the material composition remains similar.
Degradation products introduce additional chemical factors into the cell-material interface. Their presence can modify how cells respond after the original surface begins to change, including whether inflammatory responses occur. Considering degradation is therefore important when assessing a material intended to remain in contact with cells, because initial compatibility may not predict later behavior.
When cell-membrane receptors bind molecules at a material interface, they can activate intracellular signaling and cytoskeletal remodeling. These processes convert chemical recognition into physical changes such as spreading and into longer-term outcomes such as proliferation or differentiation. The connection explains why surface-bound molecules and material properties can influence cell fate together.
A useful assessment should consider the surface chemistry, charge, wettability, roughness, stiffness, and possible degradation products, then relate these features to adhesion, spreading, proliferation, differentiation, and inflammatory responses. Examining both chemical and physical variables helps distinguish which material characteristics support the intended cellular behavior and which may create compatibility concerns.
For implants and tissue-engineering scaffolds, the goal is to select surface and bulk characteristics that encourage appropriate cell attachment and subsequent behavior. Researchers can use the relationships among interfacial chemistry, physical properties, degradation, and cellular responses to improve biological compatibility and function. This approach connects material selection with outcomes such as spreading, proliferation, and differentiation.
In biosensors, cellular responses to surface-bound molecules and material properties can affect device function and biological compatibility. In drug-delivery systems, the same interfacial principles help explain how cells encounter the material and respond to it. Understanding these interactions supports designs intended to perform effectively while limiting undesirable inflammatory or compatibility-related outcomes.