Flow Injection

Flow injection is an automated analytical technique in which a sample is introduced into a continuously moving carrier stream and measured as it passes through a detection system. After injection, controlled dispersion mixes the sample with reagents in tubing, allowing chemical reactions to develop before a detector records a transient signal related to analyte concentration. In environmental analysis, flow injection supports rapid, reproducible measurements of nutrients, metals, and other contaminants in water and related samples. Its continuous operation can reduce manual handling, reagent consumption, and analysis time, making it useful for routine monitoring and high-throughput assessment of environmental quality.

Flow Injection - Related Videos

Research

JoVE Journal - Developmental Biology

In Utero Intra-cardiac Tomato-lectin Injections on Mouse Embryos to Gauge Renal Blood Flow

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Cited by 2 •

2015

This manuscript describes a technique for visualization of the developing vasculature. Here we utilized in utero intra-cardiac FITC-labeled tomato lectin microinjections on mouse embryos. Using this technique, we delineate the perfused and unperfused vessels throughout the embryonic kidney.

Mouse Round Spermatid Injection

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Cited by 1 •

2024

Here, we introduce a method for performing round spermatid injection (ROSI) in mice, a technique with promising clinical applications and utility for investigating the mechanisms underlying embryonic development.

Research

JoVE Journal - Bioengineering

Automation of the Micronucleus Assay Using Imaging Flow Cytometry and Artificial Intelligence

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Cited by 5 •

2023

The micronucleus (MN) assay is a well-established test for quantifying DNA damage. However, scoring the assay using conventional techniques such as manual microscopy or feature-based image analysis is laborious and challenging. This paper describes the methodology to develop an artificial intelligence model to score the MN assay using imaging flow cytometry data.

Flow Cytometry Purification of Mouse Meiotic Cells

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Cited by 57 •

2011

An efficient method to obtain highly purified viable meiotic fractions from mouse testis is described, which combines a refined cell dissociation protocol with fluorescent activated cell sorting (FACS). This method takes advantage of differences in the DNA content and nuclear density of discrete meiotic fractions.

Research

JoVE Journal - Cancer Research
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Facilitating Repeat Intracarotid Injections in Mouse Models by a Novel Injection Site Repair Technique

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Cited by 1 •

2024

Repair of the intracarotid artery in a mouse model after injection returns blood flow to the artery without negatively impacting the distribution of the injected material. Injection site repair facilitates subsequent injections through the same artery and prevents cerebral ischemia in mouse strains that lack a complete Circle of Willis.

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