Electron Scattering Dynamics

Electron scattering dynamics describes how electrons change direction, energy, or momentum when they interact with atoms, molecules, surfaces, or other particles. The process is governed by electromagnetic forces and can involve elastic scattering, which primarily changes an electron’s trajectory, or inelastic scattering, which transfers energy to excite atoms, generate vibrations, or release secondary electrons. Measuring scattering angles, energies, and time-dependent behavior reveals interaction cross sections and material properties. In physics, these dynamics support studies of atomic structure, plasma behavior, radiation transport, and condensed matter, while informing technologies such as electron microscopy, spectroscopy, semiconductor characterization, and particle detectors.

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JoVE EoE - Viral Growth and Techniques

Tracking Aggregation of Virus-Like Particles Using Dynamic Light Scattering

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2026

Source: Moore, M. D., et al. Alternative In Vitro Methods for the Determination of Viral Capsid Structural Integrity. J. Vis. Exp. (2017)The video demonstrates a protocol for monitoring the heat-induced aggregation of virus-like particles using dynamic light scattering (DLS). It begins with preparing a monodisperse suspension of purified particles. The sample is then loaded into a preheated DLS instrument. As the capsid proteins denature with heat, exposed hydrophobic regions cause particle...

Research

JoVE Journal - Medicine

Dynamic Light Scattering Analysis for the Determination of the Particle Size of Iron-Carbohydrate Complexes

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Cited by 2 •

2023

Dynamic light scattering (DLS) has emerged as a suitable assay for evaluating the particle size and distribution of intravenously administered iron-carbohydrate complexes. However, the protocols lack standardization and need to be modified for each iron-carbohydrate complex analyzed. The present protocol describes the application and special considerations for the analysis of iron sucrose.

Research

JoVE Journal - Biology
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Flash-and-Freeze: A Novel Technique to Capture Membrane Dynamics with Electron Microscopy

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Cited by 7 •

2017

We developed a novel technique in electron microscopy, "flash-and-freeze," that enables the visualization of membrane dynamics with ms temporal resolution. This technique combines the optogenetic stimulation of neurons with high-pressure freezing. Here, we demonstrate the procedures and describe the protocols in detail.

Revealing Dynamic Processes of Materials in Liquids Using Liquid Cell Transmission Electron Microscopy

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Cited by 9 •

2012

We have developed a self-contained liquid cell, which allows imaging through liquids using a transmission electron microscope. Dynamic processes of nanoparticles in liquids can be revealed in real time with sub-nanometer resolution.

Application of Voltage in Dynamic Light Scattering Particle Size Analysis

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Cited by 7 •

2020

Here, a protocol to apply voltage to solution during dynamic light scattering particle size measurements with the intent to explore the effect of voltage and temperature changes on polymer aggregation is presented.

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