Nanometer Spatial Resolution
Nanometer Spatial Resolution - Related Videos
10:25
Sub-nanometer Resolution Imaging with Amplitude-modulation Atomic Force Microscopy in Liquid
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Cited by 40 •
2016
We present a method for achieving sub-nanometer resolution images with amplitude-modulation (tapping mode) atomic force microscopy in liquid. The method is demonstrated on commercial atomic force microscopes. We explain the rationale behind our choices of parameters and suggest strategies for resolution optimization.
08:52
Spatial Molecular Imaging of the Glycome Using Mass Spectrometry
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Cited by 1 •
2025
This protocol details the key steps to enable rigorous and reproducible measurement of both glycogen and N-linked glycans using mass spectrometry imaging.
07:48
High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue
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Cited by 2 •
2022
Here, we present a protocol for high-resolution optical detection of chemical information around implanted medical devices with X-ray excited luminescence chemical imaging (XELCI). This novel imaging technique is developed in our lab which enables studying implant-associated infection biochemistry.
11:56
Fluorescence Imaging with One-nanometer Accuracy (FIONA)
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Cited by 11 •
2014
Single fluorophores can be localized with nanometer precision using FIONA. Here a summary of the FIONA technique is reported, and how to carry out FIONA experiments is described.
07:51
Spatial Cueing
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2023
Source: Laboratory of Jonathan Flombaum—Johns Hopkins University Attention refers to the limited human ability to select some information for processing at the expense of other stimuli in the environment. Attention operates in all sensory modalities: vision, hearing, touch, even taste and smell. It is most often studied in the visual domain though. A common way to study visual attention is with a spatial cueing paradigm. This paradigm allows researchers to measure the consequences of focusing...
FAQs
Electron microscopy obtains nanoscale structural information through electron scattering. The resulting signal can reveal organization in biological samples that is not accessible with conventional light microscopy. In bioengineering, this makes the approach useful for examining membrane architecture, protein assemblies, and interfaces where structural arrangement is central to understanding how an engineered biological system is organized.
Scanning probe microscopy uses controlled movement of a nanoscale probe across a sample. This mechanism provides a different route to spatial information than electron scattering or fluorescence localization, because the probe maps sample features through its movement. Its relevance is strongest when bioengineers need to characterize nanoscale organization at a cellular or material interface.
Super-resolution fluorescence imaging achieves nanoscale localization by precisely determining the positions of individual fluorophores. This approach can show how labeled components are arranged within biological structures, complementing structural information from electron scattering or probe-based mapping. In bioengineering, that capability supports analysis of protein assemblies and cellular interfaces at molecularly relevant scales.
These approaches should be viewed as complementary rather than interchangeable. Electron microscopy emphasizes information generated by electron scattering, scanning probe microscopy uses nanoscale probe movement, and super-resolution fluorescence imaging localizes individual fluorophores. Comparing these signal-generation strategies helps bioengineers match an imaging approach to the structure, molecular position, or interface they need to investigate.
Selection begins with the biological or engineered feature being examined. Membrane architecture and protein assemblies may call for a method that emphasizes structural organization, whereas molecular positions can be studied through fluorophore localization; cellular interfaces and biomaterials also provide distinct targets. This feature-based choice keeps the measurement aligned with the research question rather than treating all nanoscale imaging as equivalent.
In bioengineering, nanometer spatial resolution can be applied to membrane architecture, protein assemblies, biomaterials, and cellular interfaces. Studying these targets reveals how components are arranged at a scale relevant to biological organization. The resulting structural information can guide work on targeted therapeutics, biosensors, tissue scaffolds, and other engineered biological systems.
The main interpretive value is the connection between molecular structure and function. A nanoscale map helps relate the arrangement of biological components or material interfaces to how an engineered system is designed to work. That connection supports development and characterization of targeted therapeutics, biosensors, tissue scaffolds, and other systems that depend on controlled biological organization.