Spatiotemporal Icp Platform
Spatiotemporal Icp Platform - Related Videos
12:00
Spatiotemporal Mapping of Motility in Ex Vivo Preparations of the Intestines
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Cited by 26 •
2016
Recently available video recording and spatiotemporal mapping (STmap) techniques make it possible to visualize and quantify both propagating and mixing patterns of intestinal motility. The goal of this protocol is to explain the generation and analysis of STmaps using the GastroIntestinal Motility Monitoring (GIMM) system.
11:19
Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast
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Cited by 4 •
2017
The fission yeast, Schizosaccharomyces pombe is an excellent model system to study cytokinesis, the final stage in cell division. Here we describe a microscopy approach to analyze different cytokinetic events in live fission yeast cells.
08:00
Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation
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Cited by 1 •
2024
This protocol describes the application of an engineered blue-light-activated allosteric switch (LightR) domain for reversible spatiotemporal control of protein activity. Utilizing Src tyrosine kinase as a model, this study offers an elaborate protocol for developing and characterizing light-regulated Src (LightR-Src). It demonstrates the versatility of this approach across enzyme classes.
06:53
Preparation of Food Samples Using Homogenization and Microwave-Assisted Wet Acid Digestion for Multi-Element Determination with ICP-MS
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Cited by 5 •
2023
The presented protocol describes sample homogenization with a laboratory mixer, acid digestion of food samples using a mixture of 68 wt% HNO3 and 30 wt% H2O2 via microwave-assisted wet acid digestion, and multi-element determination performed with inductively coupled plasma mass spectrometry.
07:41
Video Imaging and Spatiotemporal Maps to Analyze Gastrointestinal Motility in Mice
0 Views •
Cited by 59 •
2016
This article describes a video imaging technique and high-resolution spatiotemporal mapping to identify changes in the neural regulation of colonic motility in adult mice. Subtle effects on gastrointestinal (GI) function can be detected using this approach in isolated tissue preparations to advance our understanding of GI disease.
FAQs
The platform links a signal, intervention, or measurement to both a defined location and a selected time point. This coordination allows different regions of a cell or tissue system to receive distinct cues rather than experiencing one uniform condition. Researchers can therefore examine how local responses change as biological events unfold, improving control over dynamic experimental designs.
Spatial patterning alone shows where a cue acts, while timed control shows when that cue becomes active or is measured. Combining them can distinguish location-dependent effects from time-dependent effects and reveal interactions between the two. This is valuable when investigating cell behavior, tissue organization, or changing microenvironmental responses that cannot be represented by a single static condition.
A uniform, static treatment applies the same condition broadly and maintains it without planned variation in location or time. In contrast, the Spatiotemporal ICP platform supports distinct cues across defined regions and time points. That added control can produce more precise observations of localized responses and better represent biological processes whose organization changes during an experiment.
The approach can organize biological signals or interventions according to where they act and when they are delivered or activated. It can also coordinate measurements at selected locations and time points. This flexibility supports experiments that compare regional responses, follow changes over time, or examine how cells and tissues react to controlled shifts in their surrounding microenvironment.
A general workflow begins by identifying the locations and time points relevant to the biological question. Researchers then assign the intended signals, interventions, or measurements to those conditions and coordinate their execution within the platform. Finally, they examine cell, tissue, or microenvironmental responses across the resulting spatial and temporal pattern to assess how the controlled cues affected the system.
Bioengineers would choose this approach when a uniform treatment cannot capture the spatial organization or changing timing of the process under study. It is especially relevant for experiments focused on dynamic cell behavior, tissue organization, or microenvironmental responses. The additional control can help model complex physiology and support more targeted experimental strategies.
In bioengineering, spatiotemporal control can support tissue engineering, regenerative medicine, and therapeutic design. Researchers can use the platform to study how cells organize, how tissues respond to changing cues, and how interventions might be targeted more precisely. Its value lies in connecting controlled experimental conditions with biological outcomes that vary across both location and time.