Detailed Preparation of Ni-NTA Lipid Monolayer Affinity Grids for Cryo-EM Structural Analysis of Soluble Polyhistidine-Tagged Protein Complexes
1Department of Biochemistry and Biophysics, UNC Chapel Hill School of Medicine, 2Department of Electrical and Computer Engineering, North Carolina State University, 3Department of Chemistry and Biochemistry, Division of Physical and Biological Sciences, University of California Santa Cruz, 4UNC Lineberger Comprehensive Cancer Center, UNC Chapel Hill School of Medicine
