Here, we describe the methodology used for generating L. panamensis and L. donovani strains expressing the gene for eGFP as a stable integrated transgene using the pLEXSY system. Transfected parasites were cloned by limiting dilution, and clones with the highest fluorescence intensity in both species were selected for further use in drug screening assays.

development-leishmania-species-strains-with-constitutive-expression

Institution - Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT-AIP)

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