<p class="jove_content">Here, we present a protocol for the <em>in vitro</em> selection of engineered transcriptional repressors (ETRs) with high, long-term, stable, on-target silencing efficiency and low genome-wide, off-target activity. This workflow allows for reducing an initial, complex repertoire of candidate ETRs to a short list, suitable for further evaluation in therapeutically relevant settings.</p>

in-vitro-selection-engineered-transcriptional-repressors-for-targeted

Institution - IRCCS San Raffaele Institute

Articles Published in JoVE