<p class="jove_content">In this article, a protocol for manual tyramide signal amplification (TSA) multiplex immunofluorescence (mIF) combined with image analysis and spatial analysis is described. This protocol can be used with formalin-fixed paraffin-embedded (FFPE) sections for the staining of two to six antigens per slide depending on the slide scanner available in the laboratory.</p>

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Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment

The tumor microenvironment (TME) is composed of a plethora of different cell types, such as cytotoxic immune cells and immunomodulatory cells. Depending ...

Institut de Recherche Expérimentale et Clinique (IREC)

Pôle MIRO

IREC Imaging Platform

Pôle de Pneumologie

ORL et Dermatologie (PNEU)

Division of Pneumology

Cliniques Universitaires St-Luc

Institut de Recherche Expérimentale et Clinique (IREC), Pôle MIRO

Institut de Recherche Expérimentale et Clinique (IREC), Pôle de Pneumologie, ORL et Dermatologie (PNEU)

Division of Pneumology, Cliniques Universitaires St-Luc

Université Catholique de Louvain (UCLouvain)

Institution - Université Catholique de Louvain (UCLouvain)

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