<p class="jove_content">Immunolabeling abnormal prion protein using immunohistochemistry protocols requires specific sample and anti-PrP antibody preparation methodologies. The present protocol describes the key steps in epitope demasking to ensure proper PrP immunolabeling and to minimize non-specific background staining. Also, this approach considers biosafety measures when conducting immunohistochemistry studies with the prion-infected tissues.</p>

detection-of-abnormal-prion-protein-by-immunohistochemistry

Institution - UEISPSA, National Institute for Agricultural and Veterinary Research (INIAV), Oeiras

Articles Published in JoVE