<p class="jove_content">Absolute quantification RNA sequencing (AQRNA-seq) is a technology developed to quantify the landscape of all small RNAs in biological mixtures. Here, both the library preparation and data processing steps of AQRNA-seq are demonstrated, quantifying changes in the transfer RNA (tRNA) pool in <em>Mycobacterium bovis</em> BCG during starvation-induced dormancy.</p>

AQRNA-seq for Quantifying Small RNAs

AQRNA-seq provides a direct linear relationship between sequencing read counts and small RNA copy numbers in a biological sample, thus enabling accurate ...

Institution - Singapore-MIT Alliance for Research and Technology Antimicrobial Resistance Interdisciplinary Research Group

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