This protocol provides a standardized within-chamber cutting approach to resolve the onset, peak timing, and kinetics of wound-induced volatile emissions, together with simultaneous gas-exchange measurements.

a-within-chamber-cutting-method-for-real-time-quantification-leaf

A Within-Chamber Cutting Method for Real-Time Quantification of Leaf Wounding Volatiles and Gas Exchange

Volatile organic compounds (VOCs) released by plant leaves play key roles in stress signaling, plant&#8211;atmosphere interactions, and plant defense. ...

This protocol presents a comparative method to assess stomatal and epidermal traits in two distinct species using epidermal peels, clearing techniques, and AI-assisted image analysis. The goal is to enable high-throughput, reproducible quantification of cellular structures underlying stomatal function for agricultural applications and broad plant research.

characterizing-stomatal-epidermal-traits-using-peels-clearing-ai

Characterizing Stomatal and Epidermal Traits Using Peels, Clearing, and AI-Based Image Analysis

Accurate characterization of stomatal morphology and surrounding epidermal traits is essential for understanding stomatal function. A comparative protocol ...

Assessing the anatomical differences between C3 and C4 leaf cross sections helps understand photosynthesis efficiency. This paper describes free-hand and semi-thin leaf cross sections preparation and examination, along with the caveats in the preparation for the crop species Triticum aestivum and Zea mays.

assessing-structural-traits-triticum-aestivum-zea-mays-for-c3-c4

Assessing Structural Traits in Triticum aestivum and Zea mays for C3 and C4 Photosynthetic Differentiation Using Free-hand and Semi-thin Sections

Institution - Estonian Academy of Sciences

Articles Published in JoVE