<p class="jove_content">We outline a transfection protocol for producing chimeric antigen receptor-natural killer (CAR-NK) cells targeting fungal pathogens using the non-viral Sleeping Beauty transposon system. To assess antigen-specific activation, we co-cultured the engineered cells with <em>Aspergillus fumigatus</em> germ tubes and measured IFN-&#947; secretion.</p>

engineering-chimeric-antigen-receptor-natural-killer-cells-targeting

Engineering Chimeric Antigen Receptor-Natural Killer Cells Targeting Fungal Infections Using the Non-viral Sleeping Beauty Transposon System

Chimeric antigen receptor (CAR)-based cell therapies have shown impressive efficacy in the treatment of hematological malignancies. Recently, these ...

Institution - Außenstelle Würzburg Zelluläre Immuntherapie

Articles Published in JoVE