<p class="jove_content">Indirect immunofluorescent (IIF) assays have traditionally been used for the detection of antinuclear antibodies (ANA) in human serum. The presence of these antibodies can aid in the diagnosis of systemic autoimmune rheumatic diseases (SARD). This protocol demonstrates how to effectively perform the IIF technique to accurately detect these autoantibodies.</p>

anti-nuclear-antibody-screening-using-hep-2-cells

Anti-Nuclear Antibody Screening Using HEp-2 Cells

The American College of Rheumatology position statement on ANA testing stipulates the use of IIF as the gold standard method for ANA screening1. Although ...

<p class="jove_content">Western blotting is an analytical technique used to detect specific proteins in a given sample of tissue homogenate or extract.</p>

western-blotting-sample-preparation-to-detection

Institution - EMD Chemicals Inc.

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