<p class="jove_content">A protocol is described that uses laser microdissection to isolate individual nematode tissues for RNA-sequencing. The protocol does not require species-specific genetic toolkits, allowing gene expression profiles to be compared between different species at the level of single-tissue samples.</p>

laser-microdissection-for-species-agnostic-single-tissue-applications

Laser Microdissection for Species-Agnostic Single-Tissue Applications

Single-cell methodologies have revolutionized the analysis of the transcriptomes of specific cell types. However, they often require species-specific ...

<p class="jove_content">The protocol describes a modification of the rapid Golgi method, which can be adapted to any part of the nervous system, for staining neurons in the hippocampus and medial prefrontal cortex of the rat.</p>

rapid-golgi-stain-for-dendritic-spine-visualization-hippocampus

Institution - Sacred Heart University

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