<p class="jove_content">We describe a simple immunoassay to measure the production of pro-inflammatory cytokines, such as IL-1 beta production, in patients presenting with autoinflammatory phenotypes. By activating cells in whole blood cultures with pathogen-associated molecular patterns, specifically with lipopolysaccharide, cytokine secretion can be conveniently evaluated in whole blood supernatants.</p>

accurate-simple-measurement-pro-inflammatory-cytokine-il-1-using

Accurate and Simple Measurement of the Pro-inflammatory Cytokine IL-1&beta; using a Whole Blood Stimulation Assay

Inflammatory processes resulting from the secretion of soluble mediators by immune cells, lead to various manifestations in skin, joints and other tissues ...

Translational Immunology Section

Office of Science and Technology

Institution - National Institute of Arthritis and Musculoskeletal and Skin Diseases

Articles Published in JoVE