<p class="jove_content">The present protocol allows efficient and stable delivery of fluorescent microspheres (MS) and quantum dots (QD) into the myocardium of live fish that can be tracked (traced) over time.</p>

intramyocardial-injection-for-study-cardiac-lymphatic-function

Intramyocardial Injection for the Study of Cardiac Lymphatic Function in Zebrafish

Zebrafish have proved to be an important model for studying cardiovascular formation and function during postembryonic development and regeneration. The ...

<p class="jove_content">We report messenger RNA (mRNA) electroporation as a method that permits fast and efficient expression of multiple proteins in the quail embryo model system. This method can be used to fluorescently label cells and record their in vivo movements by time-lapse microscopy shortly after electroporation.</p>

fast-efficient-expression-multiple-proteins-avian-embryos-using-mrna

Fast and Efficient Expression of Multiple Proteins in Avian Embryos Using mRNA Electroporation

We report that mRNA electroporation permits fluorescent proteins to label cells in living quail embryos more quickly and broadly than DNA electroporation. ...

Institution - Children's Hospital Los Angeles

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