Biofilms of the bacterial pathogen Clostridioides difficile and their significance in disease are not well understood. Recent findings have suggested a role in recurrence, underscoring their importance. Here, we describe the adaptation of a biofilm assay coupled to a metabolic readout with applications for drug discovery against biofilms.

biofilm-assay-for-clostridioides-difficile-with-applications-for-drug

Biofilm Assay for Clostridioides difficile with Applications for Drug Discovery

Clostridioides difficile is a gastrointestinal bacterial pathogen able to take advantage of a dysbiotic microbiota environment to proliferate, secrete ...

Here we present how to expose the geniculate ganglion of a live, anesthetized laboratory mouse and how to use calcium imaging to measure the responses of ensembles of these neurons to taste stimuli, allowing for multiple trials with different stimulants. This allows for in depth comparisons of which neurons respond to which tastants.

in-vivo-calcium-imaging-mouse-geniculate-ganglion-neuron-responses-to

In vivo Calcium Imaging of Mouse Geniculate Ganglion Neuron Responses to Taste Stimuli

Within the last ten years, advances in genetically encoded calcium indicators (GECIs) have promoted a revolution in in vivo functional imaging. Using ...

We synthesized star shaped gold nanostars using a silver seed mediated growth method. The diameter of the nanostars ranges from 200 to 300 nm and the number of tips vary from 7 to 10. The nanoparticles have a broad surface plasmon resonance mode centered in the near infrared.

gold-nanostar-synthesis-with-a-silver-seed-mediated-growth-method

Gold Nanostar Synthesis with a Silver Seed Mediated Growth Method

The physical, chemical and optical properties of nano-scale colloids depend on their material composition, size and shape 1-5. There is a great interest ...

Institution - The University of Texas at San Antonio

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