This protocol describes single-antibody labeling (SAL) to resolve the nanoscale spatial organization of plasma membrane proteins. By leveraging cumulative antibody-epitope interactions at the single-molecule level, membrane SAL (mSAL) maps local epitope distributions while simultaneously capturing antibody binding behavior in the native cellular environment.

single-molecule-localization-microscopy-membrane-proteins-using

Single-Molecule Localization Microscopy of Membrane Proteins using Single-Antibody Labeling

The plasma membrane defines the cell shape and serves as the interface that governs intercellular communication. Membrane proteins constitute a major ...

Here, we present a step-by-step, visual workflow for analyzing a single-cell time-course transcriptomics dataset of mouse skin wound healing using R. The protocol includes a standard pipeline for dataset download, quality control, visualizations, and cell type annotations using Seurat, and cell-cell interaction analysis using CellChat.

using-r-seurat-cellchat-to-analyze-single-cell-transcriptomics

Using R, Seurat, and CellChat to Analyze a Single-Cell Transcriptomics Dataset of Mouse Skin Wound Healing

The process of wound healing is regulated by complex interactions between different cell types across space and time. Through the profiling of individual ...

This protocol describes a standard intermittent-access two-bottle choice home cage drinking paradigm to model alcohol consumption in rats. In addition, it provides step-by-step instructions to augment the standard protocol with a DIY lick detection system that enables microstructural analysis of drinking behavior.

modeling-alcohol-consumption-rodents-using-two-bottle-choice-home

Modeling Alcohol Consumption in Rodents Using Two-Bottle Choice Home Cage Drinking and Microstructural Analysis

Two-bottle choice home cage drinking is one of the most widely used paradigms to study ethanol consumption in rodents. In its simplest form, animals are ...

Institution - University of Illinois Chicago

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